ArticleJournal of visualized experiments : JoVE2015
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Trimester-dependent vertical transmission of H5N1 influenza virus through placental and mammary routes impairs offspring development.Nature communications · 2026Article
- Application of a uniaxial force by pulling the skin around the mammary gland may affect the orientation of the ducts and the length of the mammary ductal network: Findings from computational modeling and laboratory experiments.PLoS computational biology · 2026Article
- Mammary γδ T cells promote IL-17A-mediated immunity againstiScience · 2023Article
- The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors.Diagnostics (Basel, Switzerland) · 2023Article
- PPAR Alpha Activation by Clofibrate Alleviates Ischemia/Reperfusion Injury in Metabolic Syndrome Rats by Decreasing Cardiac Inflammation and Remodeling and by Regulating the Atrial Natriuretic Peptide Compensatory Response.International journal of molecular sciences · 2023Article
- Severe heat stress modulated nuclear factor erythroid 2-related factor 2 and macrophage migration inhibitory factor pathway in rat liver.Journal of cell communication and signaling · 2022Article
- Dynamic miRNA Landscape Links Mammary Gland Development to the Regulation of Milk Protein Expression in Mice.Animals : an open access journal from MDPI · 2022Article
- Extracellular Vesicles in Human Milk.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Animal Models for In Vivo Lactation Studies: Anatomy, Physiology and Milk Compositions in the Most Used Non-Clinical Species: A Contribution from the ConcePTION Project.Animals : an open access journal from MDPI · 2021Review
- Insights Into Leukocyte Trafficking in Inflammatory Arthritis - Imaging the Joint.Frontiers in cell and developmental biology · 2021Review
- Got Milk? Identifying and Characterizing Lactation Defects in Genetically-Engineered Mouse Models.Journal of mammary gland biology and neoplasia · 2020Review
- Generation of Mosaic Mammary Organoids by Differential Trypsinization.Journal of visualized experiments : JoVE · 2020Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Indirect immunofluorescence is used to detect and locate proteins of interest in a tissue. The protocol presented here describes a complete and simple method for the immune detection of proteins, the mouse lactating mammary gland being taken as an example. A protocol for the preparation of the tissue samples, especially concerning the dissection of mouse mammary gland, tissue fixation and frozen tissue sectioning, are detailed. A standard protocol to perform indirect immunofluorescence, including an optional antigen retrieval step, is also presented. The observation of the labeled tissue sections as well as image acquisition and post-treatments are also stated. This procedure gives a full overview, from the collection of animal tissue to the cellular localization of a protein. Although this general method can be applied to other tissue samples, it should be adapted to each tissue/primary antibody couple studied.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.