ArticlePloS one2015
The Rickettsia Endosymbiont of Ixodes pacificus Contains All the Genes of De Novo Folate Biosynthesis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 136 citations in OpenAlex.
- Genomic Evolution of Francisella: Metabolic Innovation, Endosymbiotic Transitions to Ticks, and Biogeographic History.Genome biology and evolution · 2026Article
- Interaction between tick and host microbiotas: a four-step waltz.Parasites & vectors · 2026Review
- Infection-Mediated Shifts in the Microbial Communities of Deer-FedMicroorganisms · 2025Article
- The Microbiome and Coxiella Diversity Found in Amblyomma hebraeum and Dermacentor rhinocerinus Ticks Sampled from White Rhinoceros.Microbial ecology · 2025Article
- Prevalence of Rickettsia species phylotype G022 and Rickettsia tillamookensis in Ixodes pacificus nymphs and adults from Northern California.Ticks and tick-borne diseases · 2025Article
- A systematic review of the microbiome ofVeterinary world · 2025Review
- Whole Genome Sequencing Reveals Clade-Specific Genetic Variation in Blacklegged Ticks.Ecology and evolution · 2025Article
- Genetic and genomic variability ofISME communications · 2025Article
- The Tick Microbiome: The "Other Bacterial Players" in Tick Biocontrol.Microorganisms · 2024Review
- Metatranscriptomic investigation of singlemSystems · 2024Article
- The role of autophagy in tick-endosymbiont interactions: insights fromMicrobiology spectrum · 2024Article
- Ticks without borders: microbiome of immature neotropical tick species parasitizing migratory songbirds along northern Gulf of Mexico.Frontiers in cellular and infection microbiology · 2024Article
- Functional analysis of Rickettsia monacensis strain humboldt folA dihydrofolate reductase gene via complementation assay.Ticks and tick-borne diseases · 2023Article
- Impact of endosymbionts on tick physiology and fitness.Parasitology · 2023Review
- Microbiota in disease-transmitting vectors.Nature reviews. Microbiology · 2023Review
- The Microbiota of a Mite Prey-Predator System on Different Host Plants Are Characterized by Dysbiosis and Potential Functional Redundancy.Microbial ecology · 2023Article
- New insights into the impact of microbiome on horizontal and vertical transmission of a tick-borne pathogen.Microbiome · 2023Article
- Substantial viral and bacterial diversity at the bat-tick interface.Microbial genomics · 2023Article
- Review
- Large-Scale Sequencing ofGenes · 2022Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Ticks and other arthropods often are hosts to nutrient providing bacterial endosymbionts, which contribute to their host's fitness by supplying nutrients such as vitamins and amino acids. It has been detected, in our lab, that Ixodes pacificus is host to Rickettsia species phylotype G021. This endosymbiont is predominantly present, and 100% maternally transmitted in I. pacificus. To study roles of phylotype G021 in I. pacificus, bioinformatic and molecular approaches were carried out. MUMmer genome alignments of whole genome sequence of I. scapularis, a close relative to I. pacificus, against completely sequenced genomes of R. bellii OSU85-389, R. conorii, and R. felis, identified 8,190 unique sequences that are homologous to Rickettsia sequences in the NCBI Trace Archive. MetaCyc metabolic reconstructions revealed that all folate gene orthologues (folA, folC, folE, folKP, ptpS) required for de novo folate biosynthesis are present in the genome of Rickettsia buchneri in I. scapularis. To examine the metabolic capability of phylotype G021 in I. pacificus, genes of the folate biosynthesis pathway of the bacterium were PCR amplified using degenerate primers. BLAST searches identified that nucleotide sequences of the folA, folC, folE, folKP, and ptpS genes possess 98.6%, 98.8%, 98.9%, 98.5% and 99.0% identity respectively to the corresponding genes of Rickettsia buchneri. Phylogenetic tree constructions show that the folate genes of phylotype G021 and homologous genes from various Rickettsia species are monophyletic. This study has shown that all folate genes exist in the genome of Rickettsia species phylotype G021 and that this bacterium has the genetic capability for de novo folate synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.