Evidence map›Paper›PMID 26650483›Full record

ArticleCellular oncology (Dordrecht, Netherlands)2016

Identification of miR-139-5p as a saliva biomarker for tongue squamous cell carcinoma: a pilot study.

Mehmet Bugrahan Duz, Omer Faruk Karatas, Esra Guzel, Nesrettin Fatih Turgut, Mehmet Yilmaz, Chad J Creighton, Mustafa Ozen

Abstract read
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In one paragraph

Article in Cellular oncology (Dordrecht, Netherlands), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed, 4 pooled it
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 4 syntheses or guidelines pooled it, 110 citations in OpenAlex.

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  15. [Advances in salivary exosomal miRNAs in head and neck squamous carcinoma].Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2024
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1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Mehmet Bugrahan DuzDepartment of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey.
Omer Faruk KaratasDepartment of Molecular Biology and Genetics, Erzurum Technical University, Erzurum, Turkey.
Esra GuzelDepartment of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey.
Nesrettin Fatih TurgutDepartment of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
Mehmet YilmazDepartment of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey.
Chad J CreightonDepartment of Medicine and Dan L. Duncan Cancer Center Division of Biostatistics, Baylor College of Medicine, Houston, TX, USA.
Mustafa OzenDepartment of Medical Genetics, Istanbul University Cerrahpasa Medical School, Istanbul, Turkey. mozen@bcm.edu.
Istanbul University · TRBaylor College of Medicine · USBiruni University · TRErzurum Technical University · TR

Funding

Tumor BiologyP30CA125123 · NCI · BAYLOR COLLEGE OF MEDICINE · PI DAVID R ROWLEY · 2007 to 2026
$73.9M
NCI NIH HHS CA125123
6 · The paper itself

Abstract

backgroundOf all human oral carcinomas, 41 % are localized to the tongue. Despite considerable improvements in both diagnosis and treatment, tongue squamous cell carcinoma (TSCC) has remained one of the most lethal types of cancer. Here, we aimed at identifying a salivary microRNA (miRNA) expression signature specific for TSCC patients.

methodsTo identify putative diagnostic biomarkers, we compared the miRNA expression profiles of saliva samples from three TSCC patients and four healthy control individuals using an Agilent miRNA microarray platform (V19). Three of the differentially expressed miRNAs identified were selected for further validation using quantitative reverse-transcription PCR (qRT-PCR) in saliva samples from 25 TSCC patients and 25 healthy control individuals.

resultsThrough microarray-based expression profiling, we found that 419 miRNAs were deregulated in the saliva samples from the TSCC patients compared to those from the healthy control individuals tested. Subsequent qRT-PCR analysis revealed that the expression level of miR-139-5p was significantly reduced in the TSCC validation samples compared to the controls. Further analysis of post-operative saliva samples derived from TSCC patients revealed that the miR-139-5p expression levels had turned back to normal again. In addition, we found that miR-139-5p exhibited enough power to discriminate pre-operative TSCC patients from both normal individuals (AUC: 0.805) and post-operative TSCC patients (AUC: 0.713), thereby underscoring its diagnostic potential.

conclusionsFrom our results we conclude that saliva can be used as a feasible source for routine TSCC diagnostics and that miR-139-5p may serve as a potential biomarker for early TSCC detection.

Indexed as

AdultAgedAged, 80 and overBiomarkers, TumorCarcinoma, Squamous CellFemaleGene Expression Regulation, NeoplasticHumansMaleMicroRNAsMiddle AgedOligonucleotide Array Sequence AnalysisPilot ProjectsROC CurveSalivaTongue NeoplasmsBiomarkers, TumorMicroRNAsMIRN139 microRNA, humanBiomarkerMicroarraymiRNASalivaTongue squamous cell carcinoma

Identifiers

PMID26650483
PMCPMC13001845
OpenAlexW2191571551

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.