ArticleJournal of visualized experiments : JoVE2015
Easy and Accurate Mechano-profiling on Micropost Arrays.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Potential theranostics of circulating tumor cells and tumor-derived exosomes application in colorectal cancer.Cancer cell international · 2020Review
- Cellogram: On-the-Fly Traction Force Microscopy.Nano letters · 2019Article
- Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms.Molecular biology of the cell · 2019Article
- The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells.Molecular biology of the cell · 2019Article
- Platelet-like particles dynamically stiffen fibrin matrices and improve wound healing outcomes.Biomaterials science · 2019Article
- Elastomeric sensor surfaces for high-throughput single-cell force cytometry.Nature biomedical engineering · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell culture substrates with integrated flexible microposts enable a user to study the mechanical interactions between cells and their immediate surroundings. Particularly, cell-substrate interactions are the main interest. Today micropost arrays are a well-characterized and established method with a broad range of applications that have been published over the last decade. However, there seems to be a reservation among biologists to adapt the technique due to the lengthy and challenging process of micropost manufacture along with the lack of easily approachable software for analyzing images of cells interacting with microposts. The force read-out from microposts is surprisingly easy. A micropost acts like a spring with the cell ideally attached at its tip. Depending on size a cell applies force from its cytoskeleton through one or multiple focal adhesion points to the micropost, thus deflecting the micropost. The amount of deflection correlates directly to the applied force in direction and in magnitude. The number of microposts covered by a cell and the post deflection patterns are characteristic and allow determination of values like force per post and many biologically relevant parameters that allow "mechano-profiling" of cell phenotypes. A convenient method for mechano-profiling is described here combining the first generation of ready-to-use commercially available microposts with an in-house developed software package that is now accessible to all researchers. As a demonstration of typical application, single images of bone cancer cells were taken in bright-field microscopy for mechano-profiling of cell line models of metastasis. This combination of commercial traction force sensors and open source software for analysis allows for the first time a rapid implementation of the micropost array technique into routine lab work done by non-expert users. Furthermore, a robust and streamlined analysis process enables a user to analyze a large number of micropost images in a highly time-efficient manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.