ArticleBioMed research international2015
In Vitro Propagation, Phytochemical Analysis, and Evaluation of Free Radical Scavenging Property of Scrophularia kakudensis Franch Tissue Extracts.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Liquid Overlay-Induced Donor Plant Vigor and Initial Ammonium-Free Regrowth Medium Are Critical to the Cryopreservation ofPlants (Basel, Switzerland) · 2024Article
- Shoot Multiplication and Callus Induction ofMolecules (Basel, Switzerland) · 2021Article
- Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit.Plant cell reports · 2021Article
- Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement ofFrontiers in plant science · 2020Article
- Night Temperature Affects the Growth, Metabolism, and Photosynthetic Gene Expression inPlants (Basel, Switzerland) · 2019Article
- Comparison of pharmacological properties and phytochemical constituents of in vitro propagated and naturally occurring liverwort Lunularia cruciata.BMC complementary and alternative medicine · 2019Article
- Supplementary Light Source Affects Growth, Metabolism, and Physiology ofBioMed research international · 2019Article
- Chemical Elicitor-Induced Modulation of Antioxidant Metabolism and Enhancement of Secondary Metabolite Accumulation in Cell Suspension Cultures of Scrophularia kakudensis Franch.International journal of molecular sciences · 2016Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The current study deals with in vitro propagation, antioxidant property estimation, and assessment of acacetin content in Scrophularia kakudensis Franch. Adventitious shoot induction was achieved from the nodal explant with the highest number of adventitious shoots per explant (17.4) on Murashige and Skoog's (MS) medium fortified with 2.0 mg·L(-1) 6-benzyladenine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Maximum number of roots per plant (16.5) was noted in half strength MS medium supplemented with 0.5 mg·L(-1) IAA. The regenerated plants displayed successful survival ratio (95%) in the greenhouse. The highest content of acacetin, a pharmaceutically important flavonoid, was observed in the shoot extracts (in vitro: 32.83 µg·g(-1) FW; in vivo: 30.05 µg·g(-1) FW) followed by root extracts. Total phenol and flavonoid contents along with free radical scavenging assays revealed the occurrence of larger amount of antioxidants in shoot extract in comparison with callus and root extracts of S. kakudensis. Thus, the outcome of the present study can be highly beneficial for the germplasm conservation and commercial cultivation of S. kakudensis for therapeutic purposes.
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