ArticleScientific reports2015
Monomeric Garnet, a far-red fluorescent protein for live-cell STED imaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Bathochromic Shift via C=O to C=S Substitution: A Far-Red Fluorogen for Multiplexed FLIM with FAST Fluorogen-Activating Protein.International journal of molecular sciences · 2025Article
- A highly stable monomeric red fluorescent protein for advanced microscopy.Nature methods · 2025Article
- Far-red fluorescent genetically encoded calcium ion indicators.Nature communications · 2025Article
- Article
- Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.International journal of molecular sciences · 2023Review
- Generation of bright monomeric red fluorescent proteinsChemical science · 2022Article
- Fluorescent Probes for STED Optical Nanoscopy.Nanomaterials (Basel, Switzerland) · 2021Review
- Deciphering the Role of Positions 145 and 165 in Fluorescence Lifetime Shortening in the EGFP Variants.Biomolecules · 2020Article
- Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.Journal of physics D: Applied physics · 2020Review
- A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.Nature communications · 2020Article
- A simple empirical algorithm for optimising depletion power and resolution for dye and system specific STED imaging.Journal of microscopy · 2019Article
- Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS.Nature protocols · 2019Article
- Near-infrared STED nanoscopy with an engineered bacterial phytochrome.Nature communications · 2018Article
- Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.Nature communications · 2018Article
- Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences.FEBS open bio · 2018Article
- In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins.Scientific reports · 2017Article
- Inducing fluorescence of uranyl acetate as a dual-purpose contrast agent for correlative light-electron microscopy with nanometre precision.Scientific reports · 2017Article
- Super-resolution optical microscopy for studying membrane structure and dynamics.Journal of physics. Condensed matter : an Institute of Physics journal · 2017Review
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- Structure-guided wavelength tuning in far-red fluorescent proteins.Current opinion in structural biology · 2016Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The advancement of far-red emitting variants of the green fluorescent protein (GFP) is crucially important for imaging live cells, tissues and organisms. Despite notable efforts, far-red marker proteins still need further optimization to match the performance of their green counterparts. Here we present mGarnet, a robust monomeric marker protein with far-red fluorescence peaking at 670 nm. Thanks to its large extinction coefficient of 95,000 M(-1)cm(-1), mGarnet can be efficiently excited with 640-nm light on the red edge of its 598-nm excitation band. A large Stokes shift allows essentially the entire fluorescence emission to be collected even with 640-nm excitation, counterbalancing the lower fluorescence quantum yield of mGarnet, 9.1%, that is typical of far-red FPs. We demonstrate an excellent performance as a live-cell fusion marker in STED microscopy, using 640 nm excitation and 780 nm depletion wavelengths.
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