ArticleDiabetes2016
Monitoring C-Peptide Storage and Secretion in Islet β-Cells In Vitro and In Vivo.
Article in Diabetes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 42 citations in OpenAlex.
- Glucose-driven intra- and inter-islet beta cell synchronization in pancreatic tissue slices.Scientific reports · 2026Article
- Secretory stimuli distinctly regulate insulin secretory granule maturation through structural remodeling.Structure (London, England : 1993) · 2025Article
- Classifying Biophysical Subpopulations of Insulin Secretory Granules using Quantitative Whole Cell Structure Analysis.bioRxiv : the preprint server for biology · 2025Article
- An antireductant approach ameliorates misfolded proinsulin-induced hyperglycemia and glucose intolerance in male Akita mice.GeroScience · 2025Article
- Article
- Extrahepatic transplantation of 3D cultured stem cell-derived islet organoids on microporous scaffolds.Biomaterials science · 2023Article
- Memory IgM protects endogenous insulin from autoimmune destruction.The EMBO journal · 2021Article
- Distinct states of proinsulin misfolding in MIDY.Cellular and molecular life sciences : CMLS · 2021Article
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose.Scientific reports · 2021Article
- Study of blood glucose and insulin infusion rate in real-time in diabetic rats using an artificial pancreas system.PloS one · 2021Article
- Imaging β-Cell Function Using a Zinc-Responsive MRI Contrast Agent May Identify First Responder Islets.Frontiers in endocrinology · 2021Article
- Imaging Beta-Cell Function in the Pancreas of Non-Human Primates Using a Zinc-Sensitive MRI Contrast Agent.Frontiers in endocrinology · 2021Article
- Imaging Tissue Physiology In Vivo by Use of Metal Ion-Responsive MRI Contrast Agents.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Functional analysis of islet cells in vitro, in situ, and in vivo.Seminars in cell & developmental biology · 2020Review
- Article
- Mitochondrial Proton Leak Regulated by Cyclophilin D Elevates Insulin Secretion in Islets at Nonstimulatory Glucose Levels.Diabetes · 2020Article
- The Role of Glycation on the Aggregation Properties of IAPP.Frontiers in molecular biosciences · 2020Article
- A Newly Discovered Phenylethanoid Glycoside fromMolecules (Basel, Switzerland) · 2019Article
- Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate.Journal of visualized experiments : JoVE · 2019Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Human proinsulin with C-peptide-bearing Superfolder Green Fluorescent Protein (CpepSfGFP) has been expressed in transgenic mice, driven by the Ins1 promoter. The protein, expressed exclusively in β-cells, is processed and stored as CpepSfGFP and human insulin comprising only ∼0.04% of total islet proinsulin plus insulin, exerting no metabolic impact. The kinetics of the release of insulin and CpepSfGFP from isolated islets appear identical. Upon a single acute stimulatory challenge in vitro, fractional release of insulin does not detectably deplete islet fluorescence. In vivo, fluorescence imaging of the pancreatic surface allows, for the first time, visual assessment of pancreatic islet insulin content, and we demonstrate that CpepSfGFP visibly declines upon diabetes progression in live lepR(db/db) mice. In anesthetized mice, after intragastric or intravenous saline delivery, pancreatic CpepSfGFP (insulin) content remains undiminished. Remarkably, however, within 20 min after acute intragastric or intravenous glucose delivery (with blood glucose concentrations reaching >15 mmol/L), a small subset of islets shows rapid dispossession of a major fraction of their stored CpepSfGFP (insulin) content, whereas most islets exhibit no demonstrable loss of CpepSfGFP (insulin). These studies strongly suggest that there are "first responder" islets to an in vivo glycemic challenge, which cannot be replicated by islets in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.