Evidence map›Paper›PMID 26645680›Full record

ArticleGenome biology and evolution2015

Organellar Genomes of White Spruce (Picea glauca): Assembly and Annotation.

Shaun D Jackman, René L Warren, Ewan A Gibb, Benjamin P Vandervalk, Hamid Mohamadi, Justin Chu, Anthony Raymond, Stephen Pleasance, Robin Coope, Mark R Wildung and 5 more

Open access · goldAbstract read
In one paragraph

Article in Genome biology and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 52 citations in OpenAlex.

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  19. Complete Chloroplast Genome Sequence of an Engelmann Spruce (Microbiology resource announcements · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Shaun D JackmanCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
René L WarrenCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Ewan A GibbCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Benjamin P VandervalkCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Hamid MohamadiCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Justin ChuCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Anthony RaymondCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Stephen PleasanceCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Robin CoopeCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Mark R WildungSchool of Molecular Biosciences, Washington State University.
Carol E RitlandDepartment of Forest and Conservation Sciences, University of British Columbia, Vancouver, BC, Canada.
Jean BousquetDepartment of Forest and Environmental Genomics, Université Laval, Québec, QC, Canada.
Steven J M JonesCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada School of Computing Science, Simon Fraser University, Burnaby, BC, Canada.
Joerg BohlmannDepartment of Forest and Conservation Sciences, University of British Columbia, Vancouver, BC, Canada Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada Department of Botany, University of British Columbia, Vancouver, BC, Canada bohlmann@msl.ubc.ca ibirol@bcgsc.ca.
Inanç BirolCanada's Michael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada School of Computing Science, Simon Fraser University, Burnaby, BC, Canada Department of Computer Science, University of British Columbia, Vancouver, BC, Canada bohlmann@msl.ubc.ca ibirol@bcgsc.ca.
Canada's Michael Smith Genome Sciences Centre · CAUniversity of British Columbia · CAUniversité Laval · CAWashington State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genome sequences of the plastid and mitochondrion of white spruce (Picea glauca) were assembled from whole-genome shotgun sequencing data using ABySS. The sequencing data contained reads from both the nuclear and organellar genomes, and reads of the organellar genomes were abundant in the data as each cell harbors hundreds of mitochondria and plastids. Hence, assembly of the 123-kb plastid and 5.9-Mb mitochondrial genomes were accomplished by analyzing data sets primarily representing low coverage of the nuclear genome. The assembled organellar genomes were annotated for their coding genes, ribosomal RNA, and transfer RNA. Transcript abundances of the mitochondrial genes were quantified in three developmental tissues and five mature tissues using data from RNA-seq experiments. C-to-U RNA editing was observed in the majority of mitochondrial genes, and in four genes, editing events were noted to modify ACG codons to create cryptic AUG start codons. The informatics methodology presented in this study should prove useful to assemble organellar genomes of other plant species using whole-genome shotgun sequencing data.

Indexed as

Genome, ChloroplastGenome, MitochondrialGenome, PlantBase SequenceContig MappingMolecular Sequence AnnotationMolecular Sequence DataPiceaABySSgenome assemblygymnospermsorganellesequencingwhite spruce

Identifiers

PMID26645680
PMCPMC4758241
OpenAlexW2191563170

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.