ArticlePeerJ2015
'Degraded' RNA profiles in Arthropoda and beyond.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- The venom gland transcriptome of Tityus paraguayensis reveals a diverse array of bioactive molecules from the Brazilian Cerrado.PloS one · 2026Article
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- Evidence for the replication of a plant rhabdovirus in its arthropod mite vector.Virus research · 2025Article
- Trophic Position of the White Worm (International journal of molecular sciences · 2024Article
- Simple protocol for combined extraction of exocrine secretions and RNA in small arthropods.Biology methods & protocols · 2024Article
- Data on SSR markers and SNPs filtered from transcriptome ofData in brief · 2023Article
- Shedding light: a phylotranscriptomic perspective illuminates the origin of photosymbiosis in marine bivalves.BMC evolutionary biology · 2020Article
- Article
- Computational discovery of hidden breaks in 28S ribosomal RNAs across eukaryotes and consequences for RNA Integrity Numbers.Scientific reports · 2019Article
- The β-oxidation pathway is downregulated during diapause termination in Calanus copepods.Scientific reports · 2019Article
- RNA profile diversity across arthropoda: guidelines, methodological artifacts, and expected outcomes.Biology methods & protocols · 2018Article
- Transcriptome sequences spanning key developmental states as a resource for the study of the cestode Schistocephalus solidus, a threespine stickleback parasite.GigaScience · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The requirement for high quality/non-degraded RNA is essential for an array of molecular biology analyses. When analysing the integrity of rRNA from the barnacle Lepas anatifera (Phylum Arthropoda, Subphylum Crustacea), atypical or sub-optimal rRNA profiles that were apparently degraded were observed on a bioanalyser electropherogram. It was subsequently discovered that the rRNA was not degraded, but arose due to a 'gap deletion' (also referred to as 'hidden break') in the 28S rRNA. An apparent excision at this site caused the 28S rRNA to fragment under heat-denaturing conditions and migrate along with the 18S rRNA, superficially presenting a 'degraded' appearance. Examination of the literature showed similar observations in a small number of older studies in insects; however, reading across multiple disciplines suggests that this is a wider issue that occurs across the Animalia and beyond. The current study shows that the 28S rRNA anomaly goes far beyond insects within the Arthropoda and is widespread within this phylum. We confirm that the anomaly is associated with thermal conversion because gap-deletion patterns were observed in heat-denatured samples but not in gels with formaldehyde-denaturing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.