Evidence map›Paper›PMID 26644873›Full record

ArticleIranian journal of microbiology2015

"In-house" production of DNA size marker from a vaccinal Bacillus anthracis strain.

Mohammad Sekhavati, Keyvan Tadayon, Rainak Ghaderi, Reza Banihashemi, Ahmad Reza Jabbari, Gholamreza Shokri, Nasim Karimnasab

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Homogeneity ofIranian journal of microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mohammad SekhavatiDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Keyvan TadayonDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Rainak GhaderiDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Reza BanihashemiDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Ahmad Reza JabbariDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Gholamreza ShokriDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.
Nasim KarimnasabDepartment of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesDNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND

methodsHere a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker. RESULTS AND

conclusionThe unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.

Indexed as

Bacillus anthracis Sterne 34F2DNA size markerPCRsequencing

Identifiers

PMID26644873
PMCPMC4670467

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.