ArticleIranian journal of microbiology2015
Molecular, chemical and biological screening of soil actinomycete isolates in seeking bioactive peptide metabolites.
Article in Iranian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Research progress on clinical challenges and prevention strategies of drug-resistantFrontiers in public health · 2026Review
- Use of soil actinomycetes for pharmaceutical, food, agricultural, and environmental purposes.3 Biotech · 2022Review
- Fruit wrapping kraft coated paper promotes the isolation of actinobacteria using ex situ and in situ methods.Folia microbiologica · 2021Article
- Endophytic actinobacteria of a halophytic desert plant3 Biotech · 2020Article
- A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities.Saudi journal of biological sciences · 2019Article
- Rare actinobacteria: a potential source of bioactive polyketides and peptides.World journal of microbiology & biotechnology · 2019Article
- Culturable rare actinomycetes from Indian forest soils: Molecular and physicochemical screening for biosynthetic genes.Iranian journal of microbiology · 2018Article
- Environmental monitoring and assessment of antibacterial metabolite producing actinobacteria screened from marine sediments in south coastal regions of Karnataka, India.Environmental monitoring and assessment · 2017Article
- Antibacterial, Anticancer and Neuroprotective Activities of Rare Actinobacteria from Mangrove Forest Soils.Indian journal of microbiology · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveDue to the evolution of multidrug-resistant strains, screening of natural resources, especially actinomycetes, for new therapeutic agents discovery has become the interests of researchers. In this study, molecular, chemical and biological screening of soil actinomycetes was carried out in order to search for peptide-producing actinomycetes. MATERIALS AND
methods60 actinomycetes were isolated from soils of Iran. The isolates were subjected to molecular screening for detection NRPS (non-ribosomal peptide synthetases) gene. Phylogenic identification of NRPS containing isolates was performed. Chemical screening of the crude extracts was performed using chlorine o-dianisidine as peptide detector reagent and bioactivity of peptide producing strains was determined by antimicrobial bioassay. High pressure liquid chromatography- mass spectrometry (HPLC-MS) with UV-visible spectroscopy was performed for detection of the metabolite diversity in selected strain.
resultsAmplified NRPS adenylation gene (700 bp) was detected among 30 strains. Phylogenic identification of these isolates showed presence of rare actinomycetes genera among the isolates and 10 out of 30 strains were subjected to chemical screening. Nocardia sp. UTMC 751 showed antimicrobial activity against bacterial and fungal test pathogens. HPLC-MS and UV-visible spectroscopy results from the crude extract showed that this strain has probably the ability to produce new metabolites.
conclusionBy application of a combined approach, including molecular, chemical and bioactivity analysis, a promising strain of Nocardia sp. UTMC 751 was obtained. This strain had significant activity against Staphylococcus aureus and Pseudomonas aeruginosa. Strain Nocardia sp. UTMC 751 produce five unknown and most probably new metabolites with molecular weights of 274.2, 390.3, 415.3, 598.4 and 772.5. This strain had showed 99% similarity to Nocardia ignorata DSM 44496 T.
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Identifiers
26644870PMC4670464W2220951247What OpenQuestion holds
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