ArticleThe Journal of cell biology2015
Yeast pex1 cells contain peroxisomal ghosts that import matrix proteins upon reintroduction of Pex1.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- PEX1The Journal of biological chemistry · 2025Article
- Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.Cells · 2022Review
- Peroxisome function relies on organelle-associated mRNA translation.Science advances · 2022Article
- Peroxisome retention involves Inp1-dependent peroxisome-plasma membrane contact sites in yeast.The Journal of cell biology · 2020Article
- A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery.International journal of molecular sciences · 2019Review
- Structural Mapping of Missense Mutations in the Pex1/Pex6 Complex.International journal of molecular sciences · 2019Review
- Lipid Droplet and Peroxisome Biogenesis: Do They Go Hand-in-Hand?Frontiers in cell and developmental biology · 2019Review
- Peroxisome biogenesis, membrane contact sites, and quality control.EMBO reports · 2019Review
- Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.Scientific reports · 2018Article
- The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading.Nature communications · 2018Article
- A New Yeast Peroxin, Pex36, a Functional Homolog of Mammalian PEX16, Functions in the ER-to-Peroxisome Traffic of Peroxisomal Membrane Proteins.Journal of molecular biology · 2017Article
- Vps13-Mcp1 interact at vacuole-mitochondria interfaces and bypass ER-mitochondria contact sites.The Journal of cell biology · 2017Article
- Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins.Biochimica et biophysica acta. Molecular cell research · 2017Article
- Organelle biogenesis in the endoplasmic reticulum.Nature cell biology · 2017Review
- Review
- Transmission Electron Microscopy as a Tool for the Characterization of Soft Materials: Application and Interpretation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pex1 and Pex6 are two AAA-ATPases that play a crucial role in peroxisome biogenesis. We have characterized the ultrastructure of the Saccharomyces cerevisiae peroxisome-deficient mutants pex1 and pex6 by various high-resolution electron microscopy techniques. We observed that the cells contained peroxisomal membrane remnants, which in ultrathin cross sections generally appeared as double membrane rings. Electron tomography revealed that these structures consisted of one continuous membrane, representing an empty, flattened vesicle, which folds into a cup shape. Immunocytochemistry revealed that these structures lack peroxisomal matrix proteins but are the sole sites of the major peroxisomal membrane proteins Pex2, Pex10, Pex11, Pex13, and Pex14. Upon reintroduction of Pex1 in Pex1-deficient cells, these peroxisomal membrane remnants (ghosts) rapidly incorporated peroxisomal matrix proteins and developed into peroxisomes. Our data support earlier views that Pex1 and Pex6 play a role in peroxisomal matrix protein import.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.