ArticlePlant physiology2016
CYTOKININ OXIDASE/DEHYDROGENASE3 Maintains Cytokinin Homeostasis during Root and Nodule Development in Lotus japonicus.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it, 114 citations in OpenAlex.
- Role of cytokinins in seed development in pulses and oilseed crops: Current status and future perspective.Frontiers in genetics · 2022Pooled it
- Evolutionary Repurposing of Cytokinin Signaling in Plant Development and Symbiosis.Plants (Basel, Switzerland) · 2026Review
- The cytokinin histidine kinase receptors regulate nodulation, shoot and root development inFrontiers in plant science · 2026Article
- Phosphate deficiency reduces nodule formation through a phosphate starvation response-like protein in Phaseolus vulgaris.Plant & cell physiology · 2025Article
- Molecular Insights into the Positive Role of Soybean Nodulation by GmWRKY17.International journal of molecular sciences · 2025Article
- Unveiling the regulatory mechanisms of nodules development and quality formation in Panax notoginseng using multi-omics and MALDI-MSI.Journal of advanced research · 2025Article
- CRISPR/Cas9 editing of two adenine phosphoribosyl transferase coding genes reveals the functional specialization of adenine salvage proteins in common bean.Journal of experimental botany · 2025Article
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- GmbZIP4a/b Positively Regulate Nodule Number by Affecting Cytokinin Biosynthesis inInternational journal of molecular sciences · 2024Article
- Differential symbiotic compatibilities between rhizobium strains and cultivated and wild soybeans revealed by anatomical and transcriptome analyses.Frontiers in plant science · 2024Article
- Transcriptomic analysis implicates ABA signaling and carbon supply in the differential outgrowth of petunia axillary buds.BMC plant biology · 2023Article
- Loss-function mutants of OsCKX gene family based on CRISPR-Cas systems revealed their diversified roles in rice.The plant genome · 2023Article
- The B-type response regulator GmRR11d mediates systemic inhibition of symbiotic nodulation.Nature communications · 2022Article
- WOX family transcriptional regulators modulate cytokinin homeostasis during leaf blade development in Medicago truncatula and Nicotiana sylvestris.The Plant cell · 2022Article
- Right time, right place: The dynamic role of hormones in rhizobial infection and nodulation of legumes.Plant communications · 2022Review
- Auxin-Cytokinin Cross Talk in Somatic Embryogenesis ofPlants (Basel, Switzerland) · 2022Article
- Cytokinin drives assembly of the phyllosphere microbiome and promotes disease resistance through structural and chemical cues.The ISME journal · 2022Article
- Calcium/calmodulin-mediated microbial symbiotic interactions in plants.Frontiers in plant science · 2022Review
- Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins are required for symbiotic nodule development in legumes, and cytokinin signaling responses occur locally in nodule primordia and in developing nodules. Here, we show that the Lotus japonicus Ckx3 cytokinin oxidase/dehydrogenase gene is induced by Nod factor during the early phase of nodule initiation. At the cellular level, pCkx3::YFP reporter-gene studies revealed that the Ckx3 promoter is active during the first cortical cell divisions of the nodule primordium and in growing nodules. Cytokinin measurements in ckx3 mutants confirmed that CKX3 activity negatively regulates root cytokinin levels. Particularly, tZ and DHZ type cytokinins in both inoculated and uninoculated roots were elevated in ckx3 mutants, suggesting that these are targets for degradation by the CKX3 cytokinin oxidase/dehydrogenase. The effect of CKX3 on the positive and negative roles of cytokinin in nodule development, infection and regulation was further clarified using ckx3 insertion mutants. Phenotypic analysis indicated that ckx3 mutants have reduced nodulation, infection thread formation and root growth. We also identify a role for cytokinin in regulating nodulation and nitrogen fixation in response to nitrate as ckx3 phenotypes are exaggerated at increased nitrate levels. Together, these findings show that cytokinin accumulation is tightly regulated during nodulation in order to balance the requirement for cell divisions with negative regulatory effects of cytokinin on infection events and root development.
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