Evidence map›Paper›PMID 26643875›Full record

ReviewCirculation research2016

Multimodal SHG-2PF Imaging of Microdomain Ca2+-Contraction Coupling in Live Cardiac Myocytes.

Samir Awasthi, Leighton T Izu, Ziliang Mao, Zhong Jian, Trevor Landas, Aaron Lerner, Rafael Shimkunas, Rahwa Woldeyesus, Julie Bossuyt, Brent M Wood and 7 more

Erratum issuedOpen access · bronzeAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Circulation research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.1field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
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  8. Article
  9. Mechano-chemo-transduction in cardiac myocytes.The Journal of physiology · 2017
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 1 institution in 2 countries.

Samir AwasthiFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Leighton T IzuFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Ziliang MaoFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Zhong JianFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Trevor LandasFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Aaron LernerFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Rafael ShimkunasFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Rahwa WoldeyesusFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Julie BossuytFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Brent M WoodFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Yi-Je ChenFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Dennis L MatthewsFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Deborah K LieuFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Nipavan ChiamvimonvatFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Kit S LamFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis.
Ye Chen-IzuFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis. jwjchan@ucdavis.edu ychenizu@ucdavis.edu.
James W ChanFrom the Center for Biophotonics (S.A., Z.M., A.L., D.L.M., J.W.C.), Division of Cardiology (D.K.L., N.C., Y.C.-I.), Division of Hematology/Oncology, Department of Internal Medicine (K.S.L.), and Department of Pathology and Laboratory Medicine (J.W.C.), UC Davis School of Medicine, University of California, Davis, Sacramento; and Departments of Pharmacology (L.T.I., Z.J., T.L., J.B., B.W., Y.-J.C., Y.C.-I.), Biomedical Engineering (S.A., R.S., R.W., Y.C.-I.), Biochemistry and Molecular Medicine (K.S.L.), and Microsurgery Core (Y.-J.C.), University of California, Davis. jwjchan@ucdavis.edu ychenizu@ucdavis.edu.
Université de Yaoundé I · CM

Funding

Functional Interactions of Cardiac Ion ChannelsR01HL085844 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN · 2007 to 2025
$7.3M
Training Program In Basic & Translational Cardiovascular ScienceT32HL086350 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Martin Cadeiras, David A. Liem · 2008 to 2026
$7.1M
Translational Study of Cardiac DysfunctionR01HL085727 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN, SIRISH, PADMINI · 2008 to 2022
$5.7M
Multi-scale Systems Model of Murine Heart FailureR01HL105242 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BERS, DONALD M, MCCULLOCH, ANDREW D. · 2012 to 2016
$3.5M
Decoding the enigma of cardiac amplificationR01HL137228 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN · 2017 to 2020
$2.2M
Novel Cell-in-Gel System for Mechanotransduction Study at the Single Cell LevelR01HL123526 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN-IZU, YE · 2015 to 2018
$1.8M
Sarcomere Length Shortening and the Destabilization of the Ca2+ Control System inR01HL090880 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI IZU, LEIGHTON T. · 2009 to 2012
$1.5M
CaMKII Inhibition as a New Therapeutic Strategy for Treating Hypertension-inducedR03AG031944 · NIA · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN-IZU, YE · 2009 to 2010
$125k
Regulation of ion channels in the heartI01BX000576 · VA · VA NORTHERN CALIFORNIA HEALTH CARE SYS · PI CHIAMVIMONVAT, NIPAVAN · 2009 to 2019
–
Metabolomics study in patients post myocardial infarctionI01CX001490 · VA · VA NORTHERN CALIFORNIA HEALTH CARE SYS · PI CHIAMVIMONVAT, NIPAVAN · 2017 to 2021
–
BLRD VA I01 BX000576CSRD VA I01 CX001490NHLBI NIH HHS HL85727NHLBI NIH HHS R01 HL085727NHLBI NIH HHS R01 HL085844NHLBI NIH HHS R01 HL090880NHLBI NIH HHS R01 HL105242NHLBI NIH HHS R01HL105242NHLBI NIH HHS R01 HL123526NHLBI NIH HHS R01HL123526NHLBI NIH HHS R01 HL137228NHLBI NIH HHS R01HL85844NHLBI NIH HHS R01HL90880NHLBI NIH HHS T32 HL086350NIA NIH HHS R03 AG031944NIA NIH HHS R03AG031944
6 · The paper itself

Abstract

rationaleCardiac myocyte contraction is caused by Ca(2+) binding to troponin C, which triggers the cross-bridge power stroke and myofilament sliding in sarcomeres. Synchronized Ca(2+) release causes whole cell contraction and is readily observable with current microscopy techniques. However, it is unknown whether localized Ca(2+) release, such as Ca(2+) sparks and waves, can cause local sarcomere contraction. Contemporary imaging methods fall short of measuring microdomain Ca(2+)-contraction coupling in live cardiac myocytes.

objectiveTo develop a method for imaging sarcomere level Ca(2+)-contraction coupling in healthy and disease model cardiac myocytes. METHODS AND

resultsFreshly isolated cardiac myocytes were loaded with the Ca(2+)-indicator fluo-4. A confocal microscope equipped with a femtosecond-pulsed near-infrared laser was used to simultaneously excite second harmonic generation from A-bands of myofibrils and 2-photon fluorescence from fluo-4. Ca(2+) signals and sarcomere strain correlated in space and time with short delays. Furthermore, Ca(2+) sparks and waves caused contractions in subcellular microdomains, revealing a previously underappreciated role for these events in generating subcellular strain during diastole. Ca(2+) activity and sarcomere strain were also imaged in paced cardiac myocytes under mechanical load, revealing spontaneous Ca(2+) waves and correlated local contraction in pressure-overload-induced cardiomyopathy.

conclusionsMultimodal second harmonic generation 2-photon fluorescence microscopy enables the simultaneous observation of Ca(2+) release and mechanical strain at the subsarcomere level in living cardiac myocytes. The method benefits from the label-free nature of second harmonic generation, which allows A-bands to be imaged independently of T-tubule morphology and simultaneously with Ca(2+) indicators. Second harmonic generation 2-photon fluorescence imaging is widely applicable to the study of Ca(2+)-contraction coupling and mechanochemotransduction in both health and disease.

Indexed as

Excitation Contraction CouplingMicroscopy, ConfocalMicroscopy, Fluorescence, MultiphotonMyocardial ContractionAniline CompoundsAnimalsCardiomyopathiesDisease Models, AnimalFluorescent DyesKineticsMaleMechanotransduction, CellularMembrane MicrodomainsMiceMultimodal ImagingMyocytes, CardiacAniline CompoundsFluo 4Fluorescent DyesXanthenescalcium signalingcardiomyopathiesmechanotransduction, cellularmicroscopy, fluorescence, multiphotonmultimodal imagingmyocardial contractionsarcomeres

Identifiers

PMID26643875
PMCPMC4740258
OpenAlexW2225949616

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.