ArticleAntimicrobial agents and chemotherapy2015
Next-Generation Sequencing and Comparative Analysis of Sequential Outbreaks Caused by Multidrug-Resistant Acinetobacter baumannii at a Large Academic Burn Center.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Risk and Prognostic Factors for Bloodstream Infections Due to Clonally TransmittedInfection and drug resistance · 2025Article
- [Strengthening the development and application of precision burn medicine].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2023Article
- Article
- Evaluation ofJAC-antimicrobial resistance · 2022Article
- Methods Combining Genomic and Epidemiological Data in the Reconstruction of Transmission Trees: A Systematic Review.Pathogens (Basel, Switzerland) · 2022Review
- Co-infection of ST2BMC infectious diseases · 2021Article
- Article
- Long-Reads-Based Metagenomics in Clinical Diagnosis With a Special Focus on Fungal Infections.Frontiers in microbiology · 2021Review
- Integrating multiple genomic technologies to investigate an outbreak of carbapenemase-producing Enterobacter hormaechei.Nature communications · 2020Article
- Whole-Genome-Sequence-Based Characterization of Extensively Drug-Resistant Acinetobacter baumannii Hospital Outbreak.mSphere · 2020Article
- Population dynamics of an Escherichia coli ST131 lineage during recurrent urinary tract infection.Nature communications · 2019Article
- Patient-to-Patient Transmission of Acinetobacter baumannii Gastrointestinal Colonization in the Intensive Care Unit.Antimicrobial agents and chemotherapy · 2019Article
- Machine Learning Approaches for Epidemiological Investigations of Food-Borne Disease Outbreaks.Frontiers in microbiology · 2019Review
- Genomic epidemiology of multidrug-resistant Gram-negative organisms.Annals of the New York Academy of Sciences · 2019Review
- Emergence and Persistence of High-Risk Clones Among MDR and XDRFrontiers in microbiology · 2018Article
- Investigation of a Carbapenemase-producingAntimicrobial resistance and infection control · 2018Article
- Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis.Clinical microbiology reviews · 2017Review
- Genomic Analysis of Multidrug-Resistant Escherichia coli from North Carolina Community Hospitals: Ongoing Circulation of CTX-M-Producing ST131-Antimicrobial agents and chemotherapy · 2017Article
- Simultaneous inference of phylogenetic and transmission trees in infectious disease outbreaks.PLoS computational biology · 2017Article
- A Prolonged Outbreak of KPC-3-Producing Enterobacter cloacae and Klebsiella pneumoniae Driven by Multiple Mechanisms of Resistance Transmission at a Large Academic Burn Center.Antimicrobial agents and chemotherapy · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Next-generation sequencing (NGS) analysis has emerged as a promising molecular epidemiological method for investigating health care-associated outbreaks. Here, we used NGS to investigate a 3-year outbreak of multidrug-resistant Acinetobacter baumannii (MDRAB) at a large academic burn center. A reference genome from the index case was generated using de novo assembly of PacBio reads. Forty-six MDRAB isolates were analyzed by pulsed-field gel electrophoresis (PFGE) and sequenced using an Illumina platform. After mapping to the index case reference genome, four samples were excluded due to low coverage, leaving 42 samples for further analysis. Multilocus sequence types (MLST) and the presence of acquired resistance genes were also determined from the sequencing data. A transmission network was inferred from genomic and epidemiological data using a Bayesian framework. Based on single-nucleotide variant (SNV) differences, this MDRAB outbreak represented three sequential outbreaks caused by distinct clones. The first and second outbreaks were caused by sequence type 2 (ST2), while the third outbreak was caused by ST79. For the second outbreak, the MLST and PFGE results were discordant. However, NGS-based SNV typing detected a recombination event and consequently enabled a more accurate phylogenetic analysis. The distribution of resistance genes varied among the three outbreaks. The first- and second-outbreak strains possessed a blaOXA-23-like group, while the third-outbreak strains harbored a blaOXA-40-like group. NGS-based analysis demonstrated the superior resolution of outbreak transmission networks for MDRAB and provided insight into the mechanisms of strain diversification between sequential outbreaks through recombination.
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Registered trials
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