Evidence map›Paper›PMID 26643193›Full record

ArticleScientific reports2015

Cell culture-derived HCV cannot infect synovial fibroblasts.

Abd-Elshafy D Nadeem, Pietschmann Thomas, Müller-Ladner Ulf, Neumann Elena, A Anggakusuma, Bahgat M Mohamed, Pessler Frank, Behrendt Patrick

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 82% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Abd-Elshafy D NadeemTWINCORE, Center for Experimental and Clinical Infection Research, Institute for Experimental Infection Research, Hannover, Germany.
Pietschmann ThomasTWINCORE, Center for Experimental and Clinical Infection Research, Institute of Experimental Virology, Hannover, Germany.
Müller-Ladner UlfDepartment of Internal Medicine and Rheumatology, Justus-Liebig University of Giessen, Rheumatology and Clinical Immunology, Kerckhoff Klinik, GmbH, Bad Nauheim, Germany.
Neumann ElenaDepartment of Internal Medicine and Rheumatology, Justus-Liebig University of Giessen, Rheumatology and Clinical Immunology, Kerckhoff Klinik, GmbH, Bad Nauheim, Germany.
A AnggakusumaTWINCORE, Center for Experimental and Clinical Infection Research, Institute of Experimental Virology, Hannover, Germany.
Bahgat M MohamedTWINCORE, Center for Experimental and Clinical Infection Research, Institute for Experimental Infection Research, Hannover, Germany.
Pessler FrankTWINCORE, Center for Experimental and Clinical Infection Research, Institute for Experimental Infection Research, Hannover, Germany.
Behrendt PatrickTWINCORE, Center for Experimental and Clinical Infection Research, Institute of Experimental Virology, Hannover, Germany.
Center for Experimental and Clinical Infection Research · DEKerckhoff Klinik · DENational Water Research Center · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Worldwide 170 million individuals are infected with hepatitis C virus (HCV), up to 45 million of whom are affected by arthropathy. It is unclear whether this is due to viral infection of synovial cells or immune-mediated mechanisms. We tested the capacity of primary synovial fibroblasts to support HCV propagation. Out of the four critical HCV receptors, only CD81 was expressed to any significant extent in OASF and RASF. Consistent with this, pseudotyped HCV particles were unable to infect these cells. Permissiveness for HCV replication was investigated by transfecting cells with a subgenomic replicon of HCV encoding a luciferase reporter. OASF and RASF did not support replication of HCV, possibly due to low expression levels of miR-122. In conclusion, primary human synovial fibroblasts are unable to support propagation of HCV in vitro. HCV-related arthropathy is unlikely due to direct infection of these cells.

Indexed as

Virus ReplicationCell LineCells, CulturedFibroblastsGene ExpressionGenome, ViralHepacivirusHumansMicroRNAsReceptors, VirusRNA, MessengerSynovial MembraneViral TropismMicroRNAsMIRN122 microRNA, humanReceptors, VirusRNA, Messenger

Identifiers

PMID26643193
PMCPMC4672287
OpenAlexW2408565030

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.