ArticleRice (New York, N.Y.)2015
Down-Regulation of Cytokinin Oxidase 2 Expression Increases Tiller Number and Improves Rice Yield.
Article in Rice (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 181 citations in OpenAlex.
- Natural variation in OsMYB305 downregulating cytokinin-mediated inhibition of leaf senescence contributes to regional adaptation in rice.The New phytologist · 2026Article
- Physiological and transcriptomic analyses elucidate the effects of nitrogen form ratios on strawberry growth and development.BMC plant biology · 2026Article
- Multiplex Gene Editing and Effect Analysis of Yield, Fragrance, and Blast Resistance Genes in Rice.Genes · 2026Article
- Nitrogen deficiency restricts gall development by altering metabolic and hormonal networks inFrontiers in plant science · 2026Article
- An extra-large panicle gene ELP1 from wild rice Oryza officinalis increases grain number and grain yield.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- The transcription factor BoMYC2 negatively regulates seed size by activating cytokinin dehydrogenase BoCKX5 in Broccoli.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Knockout ofPlants (Basel, Switzerland) · 2025Article
- Introgression of genes associated with yield enhancement and resistance against bacterial leaf blight and blast diseases into an elite rice variety, Jaya.The plant genome · 2025Article
- Carbohydrate flow during grain filling: Phytohormonal regulation and genetic control in rice (Oryza sativa).Journal of integrative plant biology · 2025Review
- Variation in WIDTH OF LEAF AND GRAIN contributes to grain and leaf size by controlling LARGE2 stability in rice.The Plant cell · 2024Article
- Cytokinin and reproductive shoot architecture: bigger and better?Biochemical Society transactions · 2024Review
- Cataloging the Genetic Response: Unveiling Drought-Responsive Gene Expression in Oil Tea Camellia (Life (Basel, Switzerland) · 2024Article
- Cytokinin oxidase2-deficient mutants improve panicle and grain architecture through cytokinin accumulation and enhance drought tolerance in indica rice.Plant cell reports · 2024Article
- Natural variations of HvSRN1 modulate the spike rachis node number in barley.Plant communications · 2024Article
- Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses.PeerJ · 2024Article
- FRIZZLE PANICLE (FZP) regulates rice spikelets development through modulating cytokinin metabolism.BMC plant biology · 2023Article
- Genetic Basis of Grain Size and Weight in Rice, Wheat, and Barley.International journal of molecular sciences · 2023Review
- OsMDH12: A Peroxisomal Malate Dehydrogenase Regulating Tiller Number and Salt Tolerance in Rice.Plants (Basel, Switzerland) · 2023Article
- GhCKX14 responding to drought stress by modulating antioxi-dative enzyme activity in Gossypium hirsutum compared to CKX family genes.BMC plant biology · 2023Article
- Molecular Network for Regulation of Seed Size in Plants.International journal of molecular sciences · 2023Review
18 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCytokinins are plant-specific hormones that affect plant growth and development. The endogenous level of cytokinins in plant cells is regulated in part by irreversible degradation via cytokinin oxidase/dehydrogenase (CKX). Among the 11 rice CKXs, CKX2 has been implicated in regulation of rice grain yield.
resultsTo specifically down-regulate OsCKX2 expression, we have chosen two conserved glycosylation regions of OsCKX2 for designing artificial short hairpin RNA interference genes (shRNA-CX3 and -CX5, representing the 5' and 3' glycosylation region sequences, respectively) for transformation by the Agrobacterium-mediated method. For each construct, 5 independent transgenic lines were obtained for detailed analysis. Southern blot analysis confirmed the integration of the shRNA genes into the rice genome, and quantitative real time RT-PCR and northern blot analyses showed reduced OsCKX2 expression in the young stem of transgenic rice at varying degrees. However, the expression of other rice CKX genes, such as CKX1 and CKX3, in these transgenic lines was not altered. Transgenic rice plants grown in the greenhouse were greener and more vigorous with delayed senescence, compared to the wild type. In field experiments, both CX3 and CX5 transgenic rice plants produced more tillers (27-81 %) and grains (24-67 %) per plant and had a heavier 1000 grain weight (5-15 %) than the wild type. The increases in grain yield were highly correlated with increased tiller numbers. Consistently, insertional activation of OsCKX2 led to increased expression of CKX2 and reduced tiller number and growth in a gene-dosage dependant manner.
conclusionsTaken together, these results demonstrate that specific suppression of OsCKX2 expression through shRNA-mediated gene silencing leads to enhanced growth and productivity in rice by increasing tiller number and grain weight.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.