ArticleMicrobiome2015
A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses.
Article in Microbiome, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Longitudinal analysis of stowaway microorganisms aboard modules and cargo spacecraft of the Chinese Space Station.Microbiome · 2026Article
- Species-specific chlorine resistance and biofilm regulation by extracellular polymeric substances and quorum sensing in drinking water pipeline bacteria.Applied and environmental microbiology · 2026Article
- Viability discrimination of bacterial microbiomes in home kitchen dish sponges using propidium monoazide treatment.Environmental microbiology reports · 2024Article
- Multi-species biofilms of environmental microbiota isolated from fruit packing facilities promoted tolerance ofBiofilm · 2024Article
- DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta.Environmental microbiome · 2023Article
- Dancing the Nanopore limbo - Nanopore metagenomics from small DNA quantities for bacterial genome reconstruction.BMC genomics · 2023Article
- Astrovirology: how viruses enhance our understanding of life in the Universe.International journal of astrobiology · 2023Article
- Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater.MicrobiologyOpen · 2023Article
- Host-Associated Phages Disperse across the Extraterrestrial Analogue Antarctica.Applied and environmental microbiology · 2022Article
- Understanding the Complexities and Changes of the Astronaut Microbiome for Successful Long-Duration Space Missions.Life (Basel, Switzerland) · 2022Review
- The Isolation and Characterization of Rare Mycobiome Associated With Spacecraft Assembly Cleanrooms.Frontiers in microbiology · 2022Article
- Article
- Article
- Stable-Isotope-Informed, Genome-Resolved Metagenomics Uncovers Potential Cross-Kingdom Interactions in Rhizosphere Soil.mSphere · 2021Article
- Review
- Ammonium Removal in Aquaponics Indicates Participation of Comammox Nitrospira.Current microbiology · 2021Article
- Assembly of Bacterial Genome Sequences from Metagenomes of Spacecraft Assembly Cleanrooms.Microbiology resource announcements · 2021Article
- Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach.Microbiome · 2021Article
- A rather dry subject; investigating the study of arid-associated microbial communities.Environmental microbiome · 2020Review
- Addition of anaerobic electron acceptors to solid media did not enhance growth of 125 spacecraft bacteria under simulated low-pressure Martian conditions.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent studies posit a reciprocal dependency between the microbiomes associated with humans and indoor environments. However, none of these metagenome surveys has considered the viability of constituent microorganisms when inferring impact on human health.
resultsReported here are the results of a viability-linked metagenomics assay, which (1) unveil a remarkably complex community profile for bacteria, fungi, and viruses and (2) bolster the detection of underrepresented taxa by eliminating biases resulting from extraneous DNA. This approach enabled, for the first time ever, the elucidation of viral genomes from a cleanroom environment. Upon comparing the viable biomes and distribution of phylotypes within a cleanroom and adjoining (uncontrolled) gowning enclosure, the rigorous cleaning and stringent control countermeasures of the former were observed to select for a greater presence of anaerobes and spore-forming microflora. Sequence abundance and correlation analyses suggest that the viable indoor microbiome is influenced by both the human microbiome and the surrounding ecosystem(s).
conclusionsThe findings of this investigation constitute the literature's first ever account of the indoor metagenome derived from DNA originating solely from the potential viable microbial population. Results presented in this study should prove valuable to the conceptualization and experimental design of future studies on indoor microbiomes aimed at inferring impact on human health.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.