Evidence map›Paper›PMID 26642355›Full record

ArticleAnalytical chemistry2016

Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy.

Nikita Mukhitov, John M Spear, Scott M Stagg, Michael G Roper

Abstract read
In one paragraph

Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Single-Cell Proteomics: The Critical Role of Nanotechnology.International journal of molecular sciences · 2022
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nikita MukhitovDepartment of Chemistry and Biochemistry, Florida State University , 95 Chieftain Way, Tallahassee, Florida 32306, United States.
John M SpearInstitute of Molecular Biophysics, Florida State University , 91 Chieftain Way, Tallahassee, Florida 32306, United States.
Scott M StaggDepartment of Chemistry and Biochemistry, Florida State University , 95 Chieftain Way, Tallahassee, Florida 32306, United States.
Michael G RoperDepartment of Chemistry and Biochemistry, Florida State University , 95 Chieftain Way, Tallahassee, Florida 32306, United States.

Funding

Tools For High-Throughput High-Resolution Three Dimensional Electron MicroscopyR01GM108753 · NIGMS · FLORIDA STATE UNIVERSITY · PI STAGG, SCOTT M · 2015 to 2020
$1.6M
Purchase of a direct electron camera for the Titan-Krios at FSUS10OD018142 · OD · FLORIDA STATE UNIVERSITY · PI TAYLOR, KENNETH ALLEN · 2014 to 2014
$384k
NIGMS NIH HHS R01 GM108753NIH HHS S10 OD018142
6 · The paper itself

Abstract

A microfluidic platform is presented for preparing negatively stained grids for use in transmission electron microscopy (EM). The microfluidic device is composed of glass etched with readily fabricated features that facilitate the extraction of the grid poststaining and maintains the integrity of the sample. Utilization of this device simultaneously reduced environmental contamination on the grids and improved the homogeneity of the heavy metal stain needed to enhance visualization of biological specimens as compared to conventionally prepared EM grids. This easy-to-use EM grid preparation device provides the basis for future developments of systems with more integrated features, which will allow for high-throughput and dynamic structural biology studies.

Indexed as

Microfluidic Analytical TechniquesAnimalsCell LineMetals, HeavyMicroscopy, Electron, TransmissionModels, MolecularParticle SizePotassium Channels, Voltage-GatedRatsStaining and LabelingKcnab2 protein, ratMetals, HeavyPotassium Channels, Voltage-Gated

Identifiers

PMID26642355
PMCPMC4730115

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.