Evidence map›Paper›PMID 26642120›Full record

ArticleNew Zealand veterinary journal2016

Ovine rumen papillae biopsy via oral endoscopy; a rapid and repeatable method for serial sampling.

K M McRae, M Schultz, C G Mackintosh, G H Shackell, M F Martinez, K J Knowler, M Williams, C Ho, S N Elmes, J C McEwan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in New Zealand veterinary journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

K M McRaea AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
M Schultzb Dunedin School of Medicine, Department of Medicine , University of Otago , PO Box 56, Dunedin , 9054 , New Zealand.
C G Mackintosha AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
G H Shackella AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
M F Martineza AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
K J Knowlera AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
M Williamsb Dunedin School of Medicine, Department of Medicine , University of Otago , PO Box 56, Dunedin , 9054 , New Zealand.
C Hob Dunedin School of Medicine, Department of Medicine , University of Otago , PO Box 56, Dunedin , 9054 , New Zealand.
S N Elmesa AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
J C McEwana AgResearch , Invermay Agricultural Centre , Private Bag 50034, Mosgiel , 9053 , New Zealand.
AgResearch · NZUniversity of Otago · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsTo explore and validate the utility of rumen endoscopy for collection of rumen papillae for gene expression measurement.

methodsFour adult Coopworth ewes were fasted for either 4 or 24 hours. Animals were sedated, placed in a dorsally recumbent position at 45 degrees with the head upright, and an endoscope inserted via a tube inserted into the mouth. Biopsies of rumen papillae were taken from the ventral surface of the rumen atrium under visual guidance. Two biopsies were collected from one of the animals that had been fasted for 4 hours, and three from one of the animals that had been fasted for 24 hours. Video of the rumen atrium and reticulum was also collected. The animals recovered uneventfully. Biopsies were subsequently used for extraction and sequencing of mRNA.

resultsThe ventral surface of the rumen atrium was accessible after 4 hours off pasture, but a larger region was accessible after 24 hours of fasting. Sedation allowed access for endoscope use for around 5 to 10 minutes after which increased saliva flow was noted. Rumen papillae biopsies were easily collected, with samples from a variety of sites collected in the ∼10 minute time window. High quality RNA was obtained for stranded mRNA sequencing. Of the resulting reads, 69-70% mapped uniquely to version 3.1 of the ovine genome, and 48-49% to a known gene. The rumen mRNA profiles were consistent with a previously reported study.

conclusionsThis method for obtaining rumenal tissue was found to be rapid and resulted in no apparent short or long term effects on the animal. High quality RNA was successfully extracted and amplified from the rumen papillae biopsies, indicating that this technique could be used for future gene expression studies. The use of rumen endoscopy could be extended to collection of a variety of rumen and reticulum anatomical measurements and deposition and retrieval of small sensors from the rumen. Rumen endoscopy offers an attractive and cost effective approach to repeated rumen biopsies compared with serial slaughter or use of cannulated animals.

Indexed as

SheepAnimalsBiopsyEndoscopy, GastrointestinalFemaleRumenbiopsyendoscopygene expressionpapillaeRumensheep

Identifiers

PMID26642120
PMCPMC4867882
OpenAlexW2276849363

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.