Evidence map›Paper›PMID 26640761›Full record

ArticleEuPA open proteomics2015

Assessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure.

Gregory W Watson, Samanthi Wickramasekara, Claudia S Maier, David E Williams, Roderick H Dashwood, Emily Ho

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EuPA open proteomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Gregory W WatsonMolecular and Cellular Biology, Oregon State University, Corvallis, OR ; Biological and Population Health Sciences, Oregon State University, Corvallis, OR.
Samanthi WickramasekaraDepartment of Chemistry, Oregon State University, Corvallis, OR.
Claudia S MaierDepartment of Chemistry, Oregon State University, Corvallis, OR.
David E WilliamsEnvironmental and Molecular Toxicology, Oregon State University, Corvallis, OR ; Linus Pauling Institute, Oregon State University, Corvallis, OR.
Roderick H DashwoodCenter for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M Science Center, Houston, TX ; Department of Nutrition & Food Science, Texas A&M University, College Station, TX ; Department of Clinical Cancer Prevention, MD Anderson Cancer Center, Houston, TX ; Department of Molecular & Cellular Medicine, Texas A&M University College of Medicine, College Station, TX.
Emily HoBiological and Population Health Sciences, Oregon State University, Corvallis, OR ; Linus Pauling Institute, Oregon State University, Corvallis, OR.
Oregon State University · USThe University of Texas MD Anderson Cancer Center · US

Funding

Reducing Susceptibility to Environmental Stress Throughout the Life SpanP30ES000210 · NIEHS · OREGON STATE UNIVERSITY · PI BECKMAN, JOSEPH S · 1985 to 2015
$21.4M
Transplacental Chemoprevention of Lung Tumors and LymphomasP01CA090890 · NCI · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI DASHWOOD, RODERICK H · 2003 to 2014
$14.2M
NCI NIH HHS P01 CA090890NIEHS NIH HHS P30 ES000210
6 · The paper itself

Abstract

The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

Indexed as

mass spectrometryprostate cancersulforaphane

Identifiers

PMID26640761
PMCPMC4666544
OpenAlexW1197446917

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.