ArticleEvolutionary applications2015
Temporal variation in antibiotic environments slows down resistance evolution in pathogenic Pseudomonas aeruginosa.
Article in Evolutionary applications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- Robust antibiotic sensitization of pathogenic Pseudomonas aeruginosa via negative hysteresis in the cell envelope.Nature communications · 2026Article
- Evolutionary drivers of divergent collateral sensitivity responses during antibiotic therapy.Nature ecology & evolution · 2026Review
- Thermal modulation of transcriptional states, biofilm architecture and survival strategies inFrontiers in microbiology · 2026Article
- Review
- Pediatric tumor microenvironment: developmental dynamics and therapy resistance.Frontiers in oncology · 2025Review
- Article
- Antifungal alternation can be beneficial for durability but at the cost of generalist resistance.Communications biology · 2023Article
- Sequential antibiotic therapy in the laboratory and in the patient.Journal of the Royal Society, Interface · 2023Article
- Evaluation of a Sequential Antibiotic Treatment Regimen of Ampicillin, Ciprofloxacin and Fosfomycin againstAntibiotics (Basel, Switzerland) · 2022Article
- Resistance is futile? Mucosal immune mechanisms in the context of microbial ecology and evolution.Mucosal immunology · 2022Review
- The cost of evolved constitutiveEcology and evolution · 2021Article
- Antibiotic treatment protocols revisited: the challenges of a conclusive assessment by mathematical modelling.Journal of the Royal Society, Interface · 2021Article
- Article
- The Genomic Basis of Rapid Adaptation to Antibiotic Combination Therapy in Pseudomonas aeruginosa.Molecular biology and evolution · 2021Article
- Evolution in alternating environments with tunable interlandscape correlations.Evolution; international journal of organic evolution · 2021Article
- Evolution of drug-resistant and virulent small colonies in phenotypically diverse populations of the human fungal pathogenProceedings. Biological sciences · 2020Article
- Reciprocal antibiotic collateral sensitivity in Burkholderia multivorans.International journal of antimicrobial agents · 2020Article
- Article
- Evolutionary Approaches to Combat Antibiotic Resistance: Opportunities and Challenges for Precision Medicine.Frontiers in immunology · 2020Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibiotic resistance is a growing concern to public health. New treatment strategies may alleviate the situation by slowing down the evolution of resistance. Here, we evaluated sequential treatment protocols using two fully independent laboratory-controlled evolution experiments with the human pathogen Pseudomonas aeruginosa PA14 and two pairs of clinically relevant antibiotics (doripenem/ciprofloxacin and cefsulodin/gentamicin). Our results consistently show that the sequential application of two antibiotics decelerates resistance evolution relative to monotherapy. Sequential treatment enhanced population extinction although we applied antibiotics at sublethal dosage. In both experiments, we identified an order effect of the antibiotics used in the sequential protocol, leading to significant variation in the long-term efficacy of the tested protocols. These variations appear to be caused by asymmetric evolutionary constraints, whereby adaptation to one drug slowed down adaptation to the other drug, but not vice versa. An understanding of such asymmetric constraints may help future development of evolutionary robust treatments against infectious disease.
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