ArticleScientific reports2015
GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- PKM2 modulation regulates leukemia cell survival.Cancer & metabolism · 2026Article
- Haploid Parthenotes Have Higher Demand for Glucose and Pyruvate Compared to Normally Fertilized Embryos During their In Vitro Development.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Pyruvate kinase M2 in Alzheimer's disease: from dysregulation to therapeutic inhibition.Brain communications · 2026Review
- Hypoxia drives cervical cancer progression via OCT4/ORAI3-dependent glycolysis and CaCellular and molecular life sciences : CMLS · 2025Article
- The impact of glycolysis on ischemic stroke: from molecular mechanisms to clinical applications.Frontiers in neurology · 2025Review
- NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2.Stem cell research & therapy · 2024Article
- Glioblastoma Metabolism: Insights and Therapeutic Strategies.International journal of molecular sciences · 2023Review
- Metabolic control of induced pluripotency.Frontiers in cell and developmental biology · 2023Review
- Functions and modulation of PKM2 activity by human papillomavirus E7 oncoprotein (Review).Oncology letters · 2023Review
- GLUT3/SLC2A3 Is an Endogenous Marker of Hypoxia in Prostate Cancer Cell Lines and Patient-Derived Xenograft Tumors.Diagnostics (Basel, Switzerland) · 2022Article
- The Role of Nitric Oxide in Stem Cell Biology.Antioxidants (Basel, Switzerland) · 2022Review
- The role of m6A RNA methylation in cancer metabolism.Molecular cancer · 2022Review
- Article
- Regulation of energy metabolism in human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2021Review
- Stemness of Human Pluripotent Cells: Hypoxia-Like Response Induced by Low Nitric Oxide.Antioxidants (Basel, Switzerland) · 2021Article
- Article
- Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism.Scientific reports · 2020Article
- Microarray Analysis of Gene Expression Involved in Butyrate-Resistant Colorectal Carcinoma HCT116 Cells.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- Role of apoptosis in the hypoxic regulation of human embryonic stem cells.Clinical medicine (London, England) · 2020Article
- Shedding New Light on Cancer Metabolism: A Metabolic Tightrope Between Life and Death.Frontiers in oncology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Human embryonic stem cells (hESCs) have the capacity to differentiate into all cell types and thus have great potential for regenerative medicine. hESCs cultured at low oxygen tensions are more pluripotent and display an increased glycolytic rate but how this is regulated is unknown. This study therefore aimed to investigate the regulation of glucose metabolism in hESCs and whether this might impact OCT4 expression. In contrast to the glucose transporter GLUT1, GLUT3 was regulated by environmental oxygen and localised to hESC membranes. Silencing GLUT3 caused a reduction in glucose uptake and lactate production as well as OCT4 expression. GLUT3 and OCT4 expression were correlated suggesting that hESC self-renewal is regulated by the rate of glucose uptake. Surprisingly, PKM2, a rate limiting enzyme of glycolysis displayed a nuclear localisation in hESCs and silencing PKM2 did not alter glucose metabolism suggesting a role other than as a glycolytic enzyme. PKM2 expression was increased in hESCs cultured at 5% oxygen compared to 20% oxygen and silencing PKM2 reduced OCT4 expression highlighting a transcriptional role for PKM2 in hESCs. Together, these data demonstrate two separate mechanisms by which genes regulating glucose uptake and metabolism are involved in the hypoxic support of pluripotency in hESCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.