ArticleThe Journal of cell biology1989
A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 102 citations in OpenAlex.
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Calsequestrin, a key protein in striated muscle health and disease.Journal of muscle research and cell motility · 2021Review
- Bidirectional Ca²⁺ signaling occurs between the endoplasmic reticulum and acidic organelles.The Journal of cell biology · 2013Article
- Calcium signalling in early embryos.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.Molecular biology of the cell · 2000Article
- Large plasma membrane disruptions are rapidly resealed by Ca2+-dependent vesicle-vesicle fusion events.The Journal of cell biology · 1997Article
- Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.The Journal of cell biology · 1993Article
- Calsequestrinlike calcium-binding protein is expressed in calcium-accumulating cells of Pistia stratiotes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Polarity and reorganization of the endoplasmic reticulum during fertilization and ooplasmic segregation in the ascidian egg.The Journal of cell biology · 1993Article
- Characterization of a calsequestrin-like protein from sea-urchin eggs.The Biochemical journal · 1992Article
- Cortical localization of a calcium release channel in sea urchin eggs.The Journal of cell biology · 1992Article
- Demonstration of calcium uptake and release by sea urchin egg cortical endoplasmic reticulum.The Journal of cell biology · 1991Article
- Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs.The Journal of cell biology · 1991Article
- Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.The Journal of cell biology · 1991Article
- Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.The Journal of cell biology · 1991Article
- Intracellular Ca2+ stores in chicken Purkinje neurons: differential distribution of the low affinity-high capacity Ca2+ binding protein, calsequestrin, of Ca2+ ATPase and of the ER lumenal protein, Bip.The Journal of cell biology · 1991Article
- Simultaneous analysis of cell Ca2+ and Ca2(+)-stimulated chloride conductance in colonic epithelial cells (HT-29).Cell regulation · 1990Article
- Reassociation of cortical secretory vesicles with sea urchin egg plasma membrane: assessment of binding specificity.The Journal of membrane biology · 1990Article
- Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein.The EMBO journal · 1989Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Using an antiserum produced against a purified calsequestrin-like (CSL) protein from a microsomal fraction of sea urchin eggs, we performed light and electron microscopic immunocytochemical localizations on sea urchin eggs and embryos in the first cell cycle. The sea urchin CSL protein has been found to bind Ca++ similarly to calsequestrin, the well-characterized Ca++ storage protein in the sarcoplasmic reticulum of muscle cells. In semi-thin frozen sections of unfertilized eggs, immunofluorescent staining revealed a tubuloreticular network throughout the cytoplasm. Staining of isolated egg cortices with the CSL protein antiserum showed the presence of a submembranous polygonal, tubular network similar to ER network patterns seen in other cells and in egg cortices treated with the membrane staining dye DiIC16[3]. In frozen sections of embryos during interphase of the first cell cycle, a cytoplasmic network similar to that of the unfertilized egg was present. During mitosis, we observed a dramatic concentration of the antibody staining within the asters of the mitotic apparatus where ER is known to aggregate. Electron microscopic localization on unfertilized eggs using peroxidase-labeled secondary antibody demonstrated the presence of the CSL protein within the luminal compartment of ER-like tubules. Finally, in frozen sections of centrifugally stratified eggs, the immunofluorescent staining concentrated in the clear zone: a layer highly enriched in ER and thought to be the site of calcium release upon fertilization. This localization of a CSL protein within the ER of the egg provides evidence for the ability of this organelle to serve a Ca++ storage role in the regulation of intracellular Ca++ in nonmuscle cells in general, and in the regulation of fertilization and cell division in sea urchin eggs in particular.
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