Evidence map›Paper›PMID 26638159›Full record

ArticleStem cell reviews and reports2016

Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.

Kaitlin C Clark, Amir Kol, Salpi Shahbenderian, Jennifer L Granick, Naomi J Walker, Dori L Borjesson

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reviews and reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kaitlin C ClarkVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA.
Amir KolVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA.
Salpi ShahbenderianVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA.
Jennifer L GranickVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA.
Naomi J WalkerVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA.
Dori L BorjessonVeterinary Clinical Sciences Department, University of Minnesota, Saint Paul, MN, 55108, USA. dlborjesson@ucdavis.edu.
University of Minnesota · US

Funding

Animal Models of Infectious Diseases Training ProgramT32AI060555 · NIAID · UNIVERSITY OF CALIFORNIA DAVIS · PI MCSORLEY, STEPHEN J · 2004 to 2025
$3.3M
NIAID NIH HHS T32 AI060555
6 · The paper itself

Abstract

Mesenchymal stem cell (MSC) therapy is being increasingly used to treat dogs and horses with naturally-occurring diseases. However these animals also serve as critical large animal models for ongoing translation of cell therapy products to the human market. MSC manufacture for clinical use mandates improvement in cell culture systems to meet demands for higher MSC numbers and removal of xeno-proteins (i.e. fetal bovine serum, FBS). While serum-free media (SFM) is commercially available, its affects on MSC phenotype and immunomodulatory functions are not fully known. The objective of this study was to determine if specific MSC culture conditions, MSC expansion in HYPERFlasks® or MSC expansion in a commercially available SFM, would alter MSC proliferation, phenotype or immunomodulatory properties in vitro. MSCs cultured in HYPERFlasks® were similar in phenotype, proliferative capacity and immunomodulatory functions to MSCs grown in standard flasks however MSC yield was markedly increased. HYPERFlasks® therefore provide a viable option to generate greater cell numbers in a streamlined manner. Canine and equine MSCs expanded in SFM displayed similar proliferation, surface phenotype and inhibitory effect on lymphocyte proliferation in vitro. However, MSCs cultured in the absence of FBS secreted significantly less PGE2, and were significantly less able to inhibit IFNγ secretion by activated T-cells. Immunomodulatory functions altered by expansion in SFM were species dependent. Unlike equine MSCs, in canine adipose-derived MSCs, the inhibition of lymphocyte proliferation was not principally modulated by PGE2. The removal of FBS from both canine and equine MSC culture systems resulted in altered immunomodulatory properties in vitro and warrants further investigation prior to moving towards FBS-free culture conditions.

Indexed as

Adipose TissueAnimalsCell- and Tissue-Based TherapyCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedCulture Media, Serum-FreeDogsHorsesImmunophenotypingLymphocytesMesenchymal Stem CellsCulture Media, Serum-FreeAdipose tissueBone marrowCanineCell cultureEquineImmunomodulationLymphocyteMesenchymal stem cellsProliferationSurface protein phenotype

Identifiers

PMID26638159
PMCPMC4841858
OpenAlexW2183709910

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.