ArticleChromosoma2016
Astonishing 35S rDNA diversity in the gymnosperm species Cycas revoluta Thunb.
Article in Chromosoma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Intragenomic and Interspecific Polymorphism of 35S rDNA Internal Transcribed Spacer 1 (ITS1) inInternational journal of molecular sciences · 2026Article
- Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing.Chinese medicine · 2024Article
- Intragenomic rDNA variation - the product of concerted evolution, mutation, or something in between?Heredity · 2023Review
- Analysis of 5S rDNA Genomic Organization Through the RepeatExplorer2 Pipeline: A Simplified Protocol.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes.The Plant journal : for cell and molecular biology · 2022Article
- Identification of 45S rDNA in Passiflora using low coverage sequencing: analysis of GC content and chromosomal localization.Molecular biology reports · 2022Article
- Annotation of Siberian Larch (Plants (Basel, Switzerland) · 2022Article
- The Divergence of Chromosome Structures and 45S Ribosomal DNA Organization inPlants (Basel, Switzerland) · 2022Article
- Structure and Methylation of 35S rDNA in AllopolyploidsFrontiers in plant science · 2022Article
- An integrated DNA and RNA variant detector identifies a highly conserved three base exon in theRNA biology · 2021Article
- Analyses of the Updated "Animal rDNA Loci Database" with an Emphasis on Its New Features.International journal of molecular sciences · 2021Article
- Concerted and birth-and-death evolution of 26S ribosomal DNA in Camellia L.Annals of botany · 2021Article
- The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum.The Plant journal : for cell and molecular biology · 2020Article
- Article
- The Utility of Graph Clustering of 5S Ribosomal DNA Homoeologs in Plant Allopolyploids, Homoploid Hybrids, and Cryptic Introgressants.Frontiers in plant science · 2020Article
- Remarkable variation of ribosomal DNA organization and copy number in gnetophytes, a distinct lineage of gymnosperms.Annals of botany · 2019Article
- Cadmium-induced genome-wide DNA methylation changes in growth and oxidative metabolism in Drosophila melanogaster.BMC genomics · 2019Article
- Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum.Journal of experimental botany · 2019Article
- Unique Epigenetic Features of Ribosomal RNA Genes (rDNA) in Early Diverging Plants (Bryophytes).Frontiers in plant science · 2019Article
- Origin and parental genome characterization of the allotetraploid Stylosanthes scabra Vogel (Papilionoideae, Leguminosae), an important legume pasture crop.Annals of botany · 2018Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In all eukaryotes, the highly repeated 35S ribosomal DNA (rDNA) sequences encoding 18S-5.8S-26S ribosomal RNA (rRNA) typically show high levels of intragenomic uniformity due to homogenisation processes, leading to concerted evolution of 35S rDNA repeats. Here, we compared 35S rDNA divergence in several seed plants using next generation sequencing and a range of molecular and cytogenetic approaches. Most species showed similar 35S rDNA homogeneity indicating concerted evolution. However, Cycas revoluta exhibits an extraordinary diversity of rDNA repeats (nucleotide sequence divergence of different copies averaging 12 %), influencing both the coding and non-coding rDNA regions nearly equally. In contrast, its rRNA transcriptome was highly homogeneous suggesting that only a minority of genes (<20 %) encode functional rRNA. The most common SNPs were C > T substitutions located in symmetrical CG and CHG contexts which were also highly methylated. Both functional genes and pseudogenes appear to cluster on chromosomes. The extraordinary high levels of 35S rDNA diversity in C. revoluta, and probably other species of cycads, indicate that the frequency of repeat homogenisation has been much lower in this lineage, compared with all other land plant lineages studied. This has led to the accumulation of methylation-driven mutations and pseudogenisation. Potentially, the reduced homology between paralogs prevented their elimination by homologous recombination, resulting in long-term retention of rDNA pseudogenes in the genome.
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