Evidence map›Paper›PMID 26637929›Full record

ArticleMolecular immunology2016

Antibodies specific for Epstein-Barr virus nuclear antigen-1 cross-react with human heterogeneous nuclear ribonucleoprotein L.

J William Lindsey, Samantha L deGannes, Kimberly A Pate, Xiurong Zhao

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 35 citations in OpenAlex.

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  8. Microorganisms' Footprint in Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

J William LindseyDepartment of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA. Electronic address: john.w.lindsey@uth.tmc.edu.
Samantha L deGannesDepartment of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
Kimberly A PateDepartment of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
Xiurong ZhaoDepartment of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
The University of Texas Health Science Center at Houston · US

Funding

Endogenous Hemoglobin And Heme Detoxification After Intracerebral HemorrhageR01NS064109 · NINDS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHAO, XIURONG · 2009 to 2013
$1.6M
NINDS NIH HHS R01 NS064109NINDS NIH HHS R01-NS064109
6 · The paper itself

Abstract

Epstein-Barr virus (EBV) is associated with multiple sclerosis (MS), and antibodies to the EBV nuclear antigen-1 (EBNA-1) are consistently increased in MS patients. The hypothesis of this study is that anti-EBNA-1 antibodies cross-react with a self antigen in MS patients. We affinity purified anti-EBNA-1 antibodies from human plasma, used the anti-EBNA-1 to immunoprecipitate antigens from human brain, and identified bound antigens with mass spectrometry. Anti-EBNA-1 consistently bound heterogeneous nuclear ribonucleoprotein L (HNRNPL). We expressed both the long and short isoforms of this protein, and verified with Western blots and ELISA that the long isoform cross-reacts with EBNA-1. Immunohistochemistry demonstrated that anti-EBNA-1 bound to an antigen in the nucleus of cultured rat central nervous system cells. ELISA demonstrated the presence of antibodies to HNRNPL in the plasma of both healthy controls and MS patients, but anti-HNRNPL was not increased in MS patients. We conclude that HNRNPL is an autoantigen which cross-reacts with EBNA-1. The relevance of this autoantigen to MS and other autoimmune diseases remains to be investigated.

Indexed as

AdultAnimalsAntibodies, ViralAntigens, ViralAutoantibodiesAutoantigensBlotting, WesternCross ReactionsEnzyme-Linked Immunosorbent AssayEpstein-Barr Virus Nuclear AntigensFemaleFluorescent Antibody TechniqueHeterogeneous-Nuclear Ribonucleoprotein LHumansImmunoprecipitationMass SpectrometryAntibodies, ViralAntigens, ViralAutoantibodiesAutoantigensEBV-encoded nuclear antigen 1Epstein-Barr Virus Nuclear AntigensHeterogeneous-Nuclear Ribonucleoprotein LHNRNPL protein, humanRibonucleoproteinsAntibody cross-reactivityEpstein-Barr virusMolecular mimicryMultiple sclerosis

Identifiers

PMID26637929
PMCPMC4698215
OpenAlexW2224589828

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.