ArticleApplied and environmental microbiology2016
Cytoplasmic CopZ-Like Protein and Periplasmic Rusticyanin and AcoP Proteins as Possible Copper Resistance Determinants in Acidithiobacillus ferrooxidans ATCC 23270.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Mycobacterium marinum MMAR_0267-regulated copper utilization facilitates bacterial escape from phagolysosome.Communications biology · 2024Article
- Comparative genomics reveals intraspecific divergence ofMicrobial genomics · 2023Article
- Introducing heterotrophic iron ore bacteria as new candidates in promoting the recovery of e-waste strategic metals.World journal of microbiology & biotechnology · 2023Article
- Molecular Insights into a Novel Cu(I)-Sensitive ArsR/SmtB Family Repressor in Extremophile Acidithiobacillus caldus.Applied and environmental microbiology · 2023Article
- Molecular Insights into the Copper-Sensitive Operon Repressor in Acidithiobacillus caldus.Applied and environmental microbiology · 2021Article
- Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake.Microorganisms · 2020Article
- Determinants of Copper Resistance inGenes · 2020Article
- Sulfobacillus thermotolerans: new insights into resistance and metabolic capacities of acidophilic chemolithotrophs.Scientific reports · 2019Article
- Iron and sulfur oxidation pathways of Acidithiobacillus ferrooxidans.World journal of microbiology & biotechnology · 2019Review
- Comparative Genomic Analysis Reveals the Distribution, Organization, and Evolution of Metal Resistance Genes in the GenusApplied and environmental microbiology · 2019Article
- Inorganic Polyphosphate, Exopolyphosphatase, andArchaea (Vancouver, B.C.) · 2018Article
- Genomic characterization of a local epidemicMicrobial genomics · 2017Article
- Microbial copper resistance: importance in biohydrometallurgy.Microbial biotechnology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acidophilic organisms, such as Acidithiobacillus ferrooxidans, possess high-level resistance to copper and other metals. A. ferrooxidans contains canonical copper resistance determinants present in other bacteria, such as CopA ATPases and RND efflux pumps, but these components do not entirely explain its high metal tolerance. The aim of this study was to find other possible copper resistance determinants in this bacterium. Transcriptional expression of A. ferrooxidans genes coding for a cytoplasmic CopZ-like copper-binding chaperone and the periplasmic copper-binding proteins rusticyanin and AcoP, which form part of an iron-oxidizing supercomplex, was found to increase when the microorganism was grown in the presence of copper. All of these proteins conferred more resistance to copper when expressed heterologously in a copper-sensitive Escherichia coli strain. This effect was absent when site-directed-mutation mutants of these proteins with altered copper-binding sites were used in this metal sensitivity assay. These results strongly suggest that the three copper-binding proteins analyzed here are copper resistance determinants in this extremophile and contribute to the high-level metal resistance of this industrially important biomining bacterium.
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