ArticleAmerican journal of physiology. Heart and circulatory physiology2016
Blunted flow-mediated responses and diminished nitric oxide synthase expression in lymphatic thoracic ducts of a rat model of metabolic syndrome.
Article in American journal of physiology. Heart and circulatory physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
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- Effect of the snake venom component crotamine on lymphatic endothelial cell responses and lymph transport.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Lymphatic Clearance and Pump Function.Cold Spring Harbor perspectives in medicine · 2023Review
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- Drug-Related Lymphedema: Mysteries, Mechanisms, and Potential Therapies.Frontiers in pharmacology · 2022Review
- Lymphatic Collecting Vessel: New Perspectives on Mechanisms of Contractile Regulation and Potential Lymphatic Contractile Pathways to Target in Obesity and Metabolic Diseases.Frontiers in pharmacology · 2022Review
- Multiple aspects of lymphatic dysfunction in anFrontiers in physiology · 2022Article
- The Microvascular-Lymphatic Interface and Tissue Homeostasis: Critical Questions That Challenge Current Understanding.Journal of vascular research · 2022Review
- Lymphatic Valve Dysfunction in Western Diet-Fed Mice: New Insights Into Obesity-Induced Lymphedema.Frontiers in pharmacology · 2022Article
- Inflamed adipose tissue: A culprit underlying obesity and heart failure with preserved ejection fraction.Frontiers in immunology · 2022Review
- Myocardial Tissue Characterization in Heart Failure with Preserved Ejection Fraction: From Histopathology and Cardiac Magnetic Resonance Findings to Therapeutic Targets.International journal of molecular sciences · 2021Review
- Microvascular and lymphatic dysfunction in HFpEF and its associated comorbidities.Basic research in cardiology · 2020Review
- The Role of Lymphatic Vascular Function in Metabolic Disorders.Frontiers in physiology · 2020Review
- Entrainment of Lymphatic Contraction to Oscillatory Flow.Scientific reports · 2019Article
- Prolonged intake of desloratadine: mesenteric lymphatic vessel dysfunction and development of obesity/metabolic syndrome.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Insulin resistance disrupts cell integrity, mitochondrial function, and inflammatory signaling in lymphatic endothelium.Microcirculation (New York, N.Y. : 1994) · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Shear-dependent inhibition of lymphatic thoracic duct (TD) contractility is principally mediated by nitric oxide (NO). Endothelial dysfunction and poor NO bioavailability are hallmarks of vasculature dysfunction in states of insulin resistance and metabolic syndrome (MetSyn). We tested the hypothesis that flow-dependent regulation of lymphatic contractility is impaired under conditions of MetSyn. We utilized a 7-wk high-fructose-fed male Sprague-Dawley rat model of MetSyn and determined the stretch- and flow-dependent contractile responses in an isobaric ex vivo TD preparation. TD diameters were tracked and contractile parameters were determined in response to different transmural pressures, imposed flow, exogenous NO stimulation by S-nitro-N-acetylpenicillamine (SNAP), and inhibition of NO synthase (NOS) by l-nitro-arginine methyl ester (l-NAME) and the reactive oxygen species (ROS) scavenging molecule 4-hydroxy-tempo (tempol). Expression of endothelial NO synthase (eNOS) in TD was determined using Western blot. Approximately 25% of the normal flow-mediated inhibition of contraction frequency was lost in TDs isolated from MetSyn rats despite a comparable SNAP response. Inhibition of NOS with l-NAME abolished the differences in the shear-dependent contraction frequency regulation between control and MetSyn TDs, whereas tempol did not restore the flow responses in MetSyn TDs. We found a significant reduction in eNOS expression in MetSyn TDs suggesting that diminished NO production is partially responsible for impaired flow response. Thus our data provide the first evidence that MetSyn conditions diminish eNOS expression in TD endothelium, thereby affecting the flow-mediated changes in TD lymphatic function.
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