Evidence map›Paper›PMID 26637447›Full record

ArticleEuropean radiology2016

Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the kidney parenchyma.

Gong Wang, Qian Zhang, Zhongxiong Zhuo, Shengzheng Wu, Zheng Liu, Hongmei Xia, Kaibin Tan, Linru Zou, Ling Gan, Yunhua Gao

Abstract read
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In one paragraph

Article in European radiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. New Ultrasound Techniques Promise Further Advances in AKI and CKD.Journal of the American Society of Nephrology : JASN · 2017
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Gong WangDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Qian ZhangDepartment of Nephrology, Xinqiao Hospital, The Third Military Medical University, Chongqing, China.
Zhongxiong ZhuoDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Shengzheng WuDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Zheng LiuDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Hongmei XiaDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Kaibin TanDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Linru ZouDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Ling GanDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China.
Yunhua GaoDepartment of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing, 400037, China. xqgaoyh@outlook.com.
Army Medical University · CNXinqiao Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesBone marrow stromal cells (BMSC) transplantation proves successful in treating kidney disease and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of ultrasound targeted microbubble destruction (UTMD) on BMSCs homing ability in treated kidney tissues.

methodsRats were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability.

resultsThe results showed that the mechanical effects of UTMD would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the UTMD group post-treatment, becoming smooth 72 h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney.

conclusionsThese results confirmed that renal micro-environment changes caused by appropriate UTMD may promote BMSC homing ability toward treated kidney tissues without renal toxicity and cell damage. KEY POINTS: • This experiment showed a feasible strategy in promoting stem cell homing ability. • The treatment uses diagnostic ultrasound during enhancement with IV microbubbles. • A suitable micro-environment was important for targeted stem cell homing and retention. • The method is effective for stem cell homing to kidney diseases. • More work is required with larger animals before potential human trials.

Indexed as

AnimalsBlotting, WesternBone Marrow TransplantationEnzyme-Linked Immunosorbent AssayKidneyMesenchymal Stem CellsMicrobubblesMicroscopy, ConfocalRatsRats, Sprague-DawleyUltrasonographyBone marrow stromal cellsHomingKidneyMicrobubbleUltrasound

Identifiers

PMID26637447
OpenAlexW2187664973

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.