ArticlePloS one2015
Time to Integrate to Nest Test Evaluation in a Mouse DSS-Colitis Model.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Evaluating the analgesic effect of metamizole in the mouse model for DSS-induced acute colitis.Frontiers in physiology · 2026Article
- Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo.Nature communications · 2025Article
- Artificial intelligence generated clinical score sheets: looking at the two faces of Janus.Laboratory animal research · 2024Article
- Post craniotomy pain management in Copenhagen rat by intraperitoneal or oral dosage of Tramadol: a comparative evaluation.Scientific reports · 2023Article
- Grimace scale assessment duringFrontiers in veterinary science · 2023Article
- Dopamine Transporter Knockout Rats Show Impaired Wellbeing in a Multimodal Severity Assessment Approach.Frontiers in behavioral neuroscience · 2022Article
- Comparative Severity Assessment of Genetic, Stress-Based, and Pharmacological Mouse Models of Depression.Frontiers in behavioral neuroscience · 2022Article
- A Japanese Herbal Formula, Daikenchuto, Alleviates Experimental Colitis by Reshaping Microbial Profiles and Enhancing Group 3 Innate Lymphoid Cells.Frontiers in immunology · 2022Article
- Lidocaine and bupivacaine as part of multimodal pain management in a C57BL/6J laparotomy mouse model.Scientific reports · 2021Article
- Morphine Exacerbates Experimental Colitis-Induced Depression of Nesting in Mice.Frontiers in pain research (Lausanne, Switzerland) · 2021Article
- Automated Home-Cage Monitoring During Acute Experimental Colitis in Mice.Frontiers in neuroscience · 2021Article
- Anti-Inflammatory and Gut Microbiota Modulatory Effect ofMicroorganisms · 2020Article
- Tell-tale TINT: Does the Time to Incorporate into Nest Test Evaluate Postsurgical Pain or Welfare in Mice?Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Assessing Affective State in Laboratory Rodents to Promote Animal Welfare-What Is the Progress in Applied Refinement Research?Animals : an open access journal from MDPI · 2019Review
- Use of the Rat Grimace Scale to Evaluate Visceral Pain in a Model of Chemotherapy-Induced Mucositis.Animals : an open access journal from MDPI · 2019Article
- A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice.Scientific reports · 2019Article
- Running in the wheel: Defining individual severity levels in mice.PLoS biology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severity assessment in laboratory animals is an important issue regarding the implementation of the 3R concept into biomedical research and pivotal in current EU regulations. In mouse models of inflammatory bowel disease severity assessment is usually undertaken by clinical scoring, especially by monitoring reduction of body weight. This requires daily observance and handling of each mouse, which is time consuming, stressful for the animal and necessitates an experienced observer. The time to integrate to nest test (TINT) is an easily applicable test detecting disturbed welfare by measuring the time interval mice need to integrate nesting material to an existing nest. Here, TINT was utilized to assess severity in a mouse DSS-colitis model. TINT results depended on the group size of mice maintained per cage with most consistent time intervals measured when co-housing 4 to 5 mice. Colitis was induced with 1% or 1.5% DSS in group-housed WT and Cd14-deficient mice. Higher clinical scores and loss of body weight were detected in 1.5% compared to 1% DSS treated mice. TINT time intervals showed no dose dependent differences. However, increased clinical scores, body weight reductions, and increased TINT time intervals were detected in Cd14-/- compared to WT mice revealing mouse strain related differences. Therefore, TINT is an easily applicable method for severity assessment in a mouse colitis model detecting CD14 related differences, but not dose dependent differences. As TINT revealed most consistent results in group-housed mice, we recommend utilization as an additional method substituting clinical monitoring of the individual mouse.
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