ArticleBiophysical journal2015
The Influence of Crowding Conditions on the Thermodynamic Feasibility of Metabolic Pathways.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- Increased production of isobutanol from xylose through metabolic engineering of Saccharomyces cerevisiae overexpressing transcription factor Znf1 and exogenous genes.FEMS yeast research · 2024Article
- Optimal evaluation of energy yield and driving force in microbial metabolic pathway variants.PLoS computational biology · 2023Article
- Spatio-temporal modeling of the crowding conditions and metabolic variability in microbial communities.PLoS computational biology · 2021Article
- The influence of the crowding assumptions in biofilm simulations.PLoS computational biology · 2021Article
- Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes.Plant physiology · 2019Review
- A Lattice-Boltzmann scheme for the simulation of diffusion in intracellular crowded systems.BMC bioinformatics · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular reactions are carried out in a crowded medium where the macromolecules occupy ∼40% of the total volume. This decrease in the available volume affects the activity of the reactants. Scaled particle theory is used for the estimation of the activity coefficients of the metabolites, and thereby for the assessment of the impact of the presence of background molecules, on the estimation of the Gibbs free energy change (ΔrG) of the reactions. The lactic acid pathway and the central carbon metabolism of Actinobacillus succinogenes for the production of succinic acid from glycerol have been used as illustrative case studies. Results suggest the importance of maintaining intracellular crowded regions to favor the feasibility of a pathway that in other circumstances would be infeasible. Moreover, the crowding conditions may change the directionality of reactions and can modify the feasible range of fluxes estimated for a metabolic system compared with those obtained at standard biological conditions.
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Registered trials
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