Evidence map›Paper›PMID 26636769›Full record

ArticlePloS one2015

Upregulation of Bone Morphogenetic Protein-2 Synthesis and Consequent Collagen II Expression in Leptin-stimulated Human Chondrocytes.

Shun-Fu Chang, Rong-Ze Hsieh, Kuo-Chin Huang, Cheng Allen Chang, Fang-Yao Chiu, Hsing-Chun Kuo, Cheng-Nan Chen, Yu-Ping Su

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Shun-Fu ChangDepartment of Medical Research and Development, Chang Gung Memorial Hospital Chiayi Branch, Chiayi, Taiwan.
Rong-Ze HsiehDepartment of Medical Research and Development, Chang Gung Memorial Hospital Chiayi Branch, Chiayi, Taiwan.
Kuo-Chin HuangDepartment of Orthopaedics, Chang Gung Memorial Hospital Chiayi Branch, Chiayi, Taiwan.
Cheng Allen ChangDepartment of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
Fang-Yao ChiuDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Hsing-Chun KuoDepartment of Nursing, Chang Gung University of Science and Technology, Chiayi, Taiwan.
Cheng-Nan ChenDepartment of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan.
Yu-Ping SuDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Chiayi Chang Gung Memorial Hospital · TWChang Gung University of Science and Technology · TWNational Chiayi University · TWNational Yang Ming Chiao Tung University · TWNational Yang Ming University Hospital · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone morphogenetic proteins (BMPs) play positive roles in cartilage development, but they can barely be detected in healthy articular cartilage. However, recent evidence has indicated that BMPs could be detected in osteoarthritic and damaged cartilage and their precise roles have not been well defined. Extremely high amounts of leptin have been reported in obese individuals, which can be associated with osteoarthritis (OA) development. The aim of this study was to investigate whether BMPs could be induced in human primary chondrocytes during leptin-stimulated OA development and the underlying mechanism. We found that expression of BMP-2 mRNA, but not BMP-4, BMP-6, or BMP-7 mRNA, could be increased in human primary chondrocytes under leptin stimulation. Moreover, this BMP-2 induction was mediated through transcription factor-signal transducer and activator of transcription (STAT) 3 activation via JAK2-ERK1/2-induced Ser727-phosphorylation. Of note, histone deacetylases (HDACs) 3 and 4 were both involved in modulating leptin-induced BMP-2 mRNA expression through different pathways: HDAC3, but not HDAC4, associated with STAT3 to form a complex. Our results further demonstrated that the role of BMP-2 induction under leptin stimulation is to increase collagen II expression. The findings in this study provide new insights into the regulatory mechanism of BMP-2 induction in leptin-stimulated chondrocytes and suggest that BMP-2 may play a reparative role in regulating leptin-induced OA development.

Indexed as

AgedAged, 80 and overBone Morphogenetic Protein 2Cells, CulturedChondrocytesCollagen Type IIFemaleGene Expression RegulationHistone Deacetylase 3Histone DeacetylasesHumansJanus Kinase 2LeptinMaleMAP Kinase Signaling SystemMiddle AgedBMP2 protein, humanBone Morphogenetic Protein 2Collagen Type IIHDAC4 protein, humanHistone Deacetylase 3Histone DeacetylasesJAK2 protein, humanJanus Kinase 2LeptinMAPK1 protein, humanMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Repressor ProteinsRNA, MessengerSTAT3 protein, humanSTAT3 Transcription Factor

Identifiers

PMID26636769
PMCPMC4670096
OpenAlexW2288384148

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.