ArticlePloS one2015
Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Evaluating paratransgenesis using engineered symbiotic bacteria for Plasmodium inhibition in mosquito vectors: A systematic review.PLoS neglected tropical diseases · 2026Pooled it
- Advances in mosquito gut microbiota: Mechanisms of regulating pathogen transmission and applications in vector control.Virulence · 2026Review
- The Adult Aedes aegypti Bacterial Microbiota is Robust to Infection with the Obligate Microsporidian Parasite Edhazardia aedis.Environmental microbiology · 2026Article
- LaboratoryFrontiers in cellular and infection microbiology · 2026Article
- Environmental persistence and transmission dynamics ofApplied and environmental microbiology · 2025Article
- Exploiting venom toxins in paratransgenesis to prevent mosquito-borne disease.Parasites & vectors · 2025Review
- Article
- Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.European journal of public health · 2025Article
- Insect‒microbe symbiosis-based strategies offer a new avenue for the management of insect pests and their transmitted pathogens.Crop health · 2024Review
- Immune Reactions of Vector Insects to Parasites and Pathogens.Microorganisms · 2024Review
- Insect Microbial Symbionts: Ecology, Interactions, and Biological Significance.Microorganisms · 2023Review
- Rodents as vehicle for delivery of transgenic bacteria to make paratransgenic sand fly vectors of cutaneous leishmaniasis in field condition.Scientific reports · 2023Article
- Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence.Insects · 2023Review
- Holobiont perspectives on tripartite interactions among microbiota, mosquitoes, and pathogens.The ISME journal · 2023Review
- Bacterial Microbiota from Lab-Reared and Field-CapturedMicroorganisms · 2023Article
- Review
- Recovery of metagenomic data from theWellcome open research · 2023Article
- Comparative analysis of the gut microbiota of sand fly vectors of zoonotic visceral leishmaniasis (ZVL) in Iran; host-environment interplay shapes diversity.PLoS neglected tropical diseases · 2022Article
- Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors.Parasites & vectors · 2022Review
- Evaluation of anti-malaria potency of wild and genetically modified Enterobacter cloacae expressing effector proteins in Anopheles stephensi.Parasites & vectors · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Novel interventions are needed to prevent the transmission of the Plasmodium parasites that cause malaria. One possible method is to supply mosquitoes with antiplasmodial effector proteins from bacteria by paratransgenesis. Mosquitoes have a diverse complement of midgut microbiota including the Gram-negative bacteria Asaia bogorensis. This study presents the first use of Asaia sp. bacteria for paratransgenesis against P. berghei. We identified putative secreted proteins from A. bogorensis by a genetic screen using alkaline phosphatase gene fusions. Two were secreted efficiently: a siderophore receptor protein and a YVTN beta-propeller repeat protein. The siderophore receptor gene was fused with antiplasmodial effector genes including the scorpine antimicrobial peptide and an anti-Pbs21 scFv-Shiva1 immunotoxin. Asaia SF2.1 secreting these fusion proteins were fed to mosquitoes and challenged with Plasmodium berghei-infected blood. With each of these effector constructs, significant inhibition of parasite development was observed. These results provide a novel and promising intervention against malaria transmission.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.