Evidence map›Paper›PMID 26636134›Full record

ArticleHSOA journal of ophthalmology & clinical research2014

Deletion of SPARC Enhances Retinal Vaso-Obliteration in Mouse Model of Oxygen-Induced Retinopathy.

Doaa Sobeih, Khaled A Hussein, Neveen Said, Kouros Motamed, Mohamed Al-Shabrawey

Open access · diamondAbstract read
PubMed Full text at PMC
In one paragraph

Article in HSOA journal of ophthalmology & clinical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Doaa SobeihOral Biology/Anatomy, College of Dental Medicine, Georgia Regents University (GRU), Augusta, GA, USA ; James & Jean Culver Vision Discovery Institute, GRU, USA ; Ophthalmology, Medical College of Georgia (MCG), GRU, USA.
Khaled A HusseinOral Biology/Anatomy, College of Dental Medicine, Georgia Regents University (GRU), Augusta, GA, USA ; James & Jean Culver Vision Discovery Institute, GRU, USA ; Ophthalmology, Medical College of Georgia (MCG), GRU, USA.
Neveen SaidDepartment of Radiation Oncology, University of Virginia School of Medicine, USA.
Kouros MotamedIgdrasol, Irivin, CA, USA.
Mohamed Al-ShabraweyOral Biology/Anatomy, College of Dental Medicine, Georgia Regents University (GRU), Augusta, GA, USA ; James & Jean Culver Vision Discovery Institute, GRU, USA ; Ophthalmology, Medical College of Georgia (MCG), GRU, USA ; Cellular Biology and Anatomy, MCG, GRU, USA.
Augusta University · US

Funding

Role of lipoxygenase pathway in early microvascular dysfunction during diabetic rR01EY023315 · NEI · AUGUSTA UNIVERSITY · PI AL-SHABRAWEY, MOHAMED AL-SAYED · 2013 to 2017
$1.8M
NEI NIH HHS R01 EY023315
6 · The paper itself

Abstract

backgroundSecreted Protein Acidic and Rich in Cysteine (SPARC) is a matricellular protein which is implicated in regulation of angiogenesis. PURPOSE: To characterize the changes in SPARC expression and effect of its deletion in a mouse model Oxygen Induced Retinopathy (OIR). MATERIALS AND

methodsWild type (wt) and SPARC-deficient mice were subjected to high oxygen (75%) for 5 days (p7-p12) before room air for additional 5 days (p12-p17). Retinas from both groups were flat mounted and retinal vessels were labeled with Isolectin-B4. Areas of Retinal Neovascularization (RNV) and vaso-obliteration were measured by Image-J and normalized to total retinal areas. SPARC expression was analyzed in both groups at p14 and p17 in retinal homogenates and sections by Western Blotting (WB) and immunofluorescence respectively. Human Retinal Endothelial Cells (HRECs) were exposed to hypoxia (1% O2) for 6 hours then SPARC was measured in cell lysate and condition medium by WB and ELISA. Moreover, HRECs were treated with VEGF or SPARC to study their mutual regulatory effect.

resultsSPARC-deficient mice demonstrated significant increase in the vaso-obliteration (

conclusionSPARC deletion enhances ischemic retinopathy, thus modulation of SPARC expression could be a novel therapeutic approach to prevent pathological RNV.

Identifiers

PMID26636134
PMCPMC4665627
OpenAlexW2184274492

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.