ArticleStem cells international2016
Improvement in Isolation and Identification of Mouse Oogonial Stem Cells.
Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Female germline stem cells: recent advances, opportunities, and challenges to overcome.Cell regeneration (London, England) · 2025Review
- Comparison of differentiation potential of ovarian surface epithelial stem cells into Oocyte-like cells between human- and mice-derived cells.American journal of stem cells · 2025Article
- Mesenchymal stem cells: Emerging concepts and recent advances in their roles in organismal homeostasis and therapy.Frontiers in cellular and infection microbiology · 2023Review
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation.Cell & bioscience · 2022Article
- Germ cells of the mammalian female: A limited or renewable resource?†.Biology of reproduction · 2021Review
- Spermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression.Cell & bioscience · 2021Article
- GAS5/miR-21 Axis as a Potential Target to Rescue ZCL-082-Induced Autophagy of Female Germline Stem Cells In Vitro.Molecular therapy. Nucleic acids · 2019Article
- Extracellular matrix signaling activates differentiation of adult ovary-derived oogonial stem cells in a species-specific manner.Fertility and sterility · 2019Article
- Review
- Female Fertility Preservation through Stem Cell-based Ovarian Tissue Reconstitution In Vitro and Ovarian Regeneration In Vivo.Clinical medicine insights. Reproductive health · 2019Review
- Cadherin 22 participates in the self-renewal of mouse female germ line stem cells via interaction with JAK2 and β-catenin.Cellular and molecular life sciences : CMLS · 2018Article
- Ovarian regeneration: The potential for stem cell contribution in the postnatal ovary to sustained endocrine function.Molecular and cellular endocrinology · 2017Review
- Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits.Journal of ovarian research · 2016Review
- Human GV oocytes generated by mitotically active germ cells obtained from follicular aspirates.Scientific reports · 2016Article
- From primordial germ cells to primordial follicles: a review and visual representation of early ovarian development in mice.Journal of ovarian research · 2016Review
- FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells.Scientific reports · 2016Article
- Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Female germline stem cells (FGSCs) or oogonial stem cells (OSCs) have the capacity to generate newborn oocytes and thus open a new door to fight ovarian aging and female infertility. However, the production and identification of OSCs are difficult for investigators. Rare amount of these cells in the ovary results in the failure of the acquisition of OSCs. Furthermore, the oocyte formation by OSCs in vivo was usually confirmed using tissue sections by immunofluorescence or immunohistochemistry in previous studies. STO or MEF feeder cells are derived from mouse, not human. In our study, we modified the protocol. The cells were digested from ovaries and cultured for 2-3 days and then were purified by magnetic-activated cell sorting (MACS). The ovaries and fetus of mice injected with EGFP-positive OSCs were prepared and put on the slides to directly visualize oocyte and progeny formation under microscope. Additionally, the human umbilical cord mesenchymal stem cells (hUC-MSCs) were also used as feeder cells to support the proliferation of OSCs. The results showed that all the modified procedures can significantly improve and facilitate the generation and characterization of OSCs, and hUC-MSCs as feeder will be useful for isolation and proliferation of human OSCs avoiding contamination from mouse.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.