ArticleFrontiers in plant science2015
Induction of Embryogenesis in Brassica Napus Microspores Produces a Callosic Subintinal Layer and Abnormal Cell Walls with Altered Levels of Callose and Cellulose.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- Transcriptomic analyses reveal regulatory plasticity and metabolic reprogramming underlying genotype-specific microspore embryogenesis in wheat.Plant cell reports · 2026Article
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Calcium levels modulate embryo yield inFrontiers in plant science · 2024Article
- Review
- The Highly EmbryogenicPlants (Basel, Switzerland) · 2023Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- Article
- Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticaleFrontiers in plant science · 2022Article
- Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores inFrontiers in plant science · 2021Article
- Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route.Plants (Basel, Switzerland) · 2020Article
- Changes of cell wall components during embryogenesis of Castanea mollissima.Journal of plant research · 2020Article
- Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.Journal of experimental botany · 2019Article
- Dynamic Changes in Arabinogalactan-Protein, Pectin, Xyloglucan and Xylan Composition of the Cell Wall During Microspore Embryogenesis inFrontiers in plant science · 2019Article
- Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment.Frontiers in plant science · 2019Review
- Microspore embryogenesis in Brassica: calcium signaling, epigenetic modification, and programmed cell death.Planta · 2018Review
- Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- Dynamics of Calcium duringFrontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this work, we used high pressure freezing and freeze substitution, immunolocalization, confocal, and electron microscopy to analyze the structure and composition of the first cell walls formed during conventional Brassica napus microspore embryogenesis, and in cultures treated to alter the intracellular Ca(2+) levels. Our results revealed that one of the first signs of embryogenic commitment is the formation of a callose-rich, cellulose-deficient layer beneath the intine (the subintinal layer), and of irregular, incomplete cell walls. In these events, Ca(2+) may have a role. We propose that abnormal cell walls are due to a massive callose synthesis and deposition of excreted cytoplasmic material, and the parallel inhibition of cellulose synthesis. These features were absent in pollen-like structures and in microspore-derived embryos, few days after the end of the heat shock, where abnormal cell walls were no longer produced. Together, our results provide an explanation to a series of relevant aspects of microspore embryogenesis including the role of Ca(2+) and the occurrence of abnormal cell walls. In addition, our discovery may be the explanation to why nuclear fusions take place during microspore embryogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.