ArticleFrontiers in microbiology2015
Identification of a Ligand Binding Pocket in LdtR from Liberibacter asiaticus.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Thermal shift assay to identify ligands for bacterial sensor proteins.FEMS microbiology reviews · 2025Review
- Recent progress in the understanding of Citrus Huanglongbing: from the perspective of pathogen and citrus host.Molecular breeding : new strategies in plant improvement · 2024Article
- Evaluation ofMolecules (Basel, Switzerland) · 2022Article
- Osmotic stress induces long-term biofilm survival in Liberibacter crescens.BMC microbiology · 2022Article
- Defeating Huanglongbing PathogenFrontiers in plant science · 2021Article
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Ferredoxin-Like Protein FerR Regulates PrbP Activity inApplied and environmental microbiology · 2019Article
- Article
- Purification and partial characterization of LdtP, a cell envelope modifying enzyme in Liberibacter asiaticus.BMC microbiology · 2018Article
- Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges.International journal of molecular sciences · 2018Review
- Transcriptome sequencing and ITRAQ reveal the detoxification mechanism of Bacillus GJ1, a potential biocontrol agent for Huanglongbing.PloS one · 2018Article
- Zinc is an inhibitor of the LdtR transcriptional activator.PloS one · 2018Article
- LdtR is a master regulator of gene expression in Liberibacter asiaticus.Microbial biotechnology · 2017Article
- Functional characterization of LotP from Liberibacter asiaticus.Microbial biotechnology · 2017Article
- Identification of the Genes Required for the Culture of Liberibacter crescens, the Closest Cultured Relative of the Liberibacter Plant Pathogens.Frontiers in microbiology · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LdtR is a transcriptional activator involved in the regulation of a putative L,D transpeptidase in Liberibacter asiaticus, an unculturable pathogen and one of the causative agents of Huanglongbing disease. Using small molecule screens we identified benzbromarone as an inhibitor of LdtR activity, which was confirmed using in vivo and in vitro assays. Based on these previous results, the objective of this work was to identify the LdtR ligand binding pocket and characterize its interactions with benzbromarone. A structural model of LdtR was constructed and the molecular interactions with the ligand were predicted using the SwissDock interface. Using site-directed mutagenesis, these residues were changed to alanine. Electrophoretic mobility shift assays, thermal denaturation, isothermal titration calorimetry experiments, and in vivo assays were used to identify residues T43, L61, and F64 in the Benz1 pocket of LdtR as the amino acids most likely involved in the binding to benzbromarone. These results provide new information on the binding mechanism of LdtR to a modulatory molecule and provide a blue print for the design of therapeutics for other members of the MarR family of transcriptional regulators involved in pathogenicity.
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