ArticleNature communications2015
Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
127 citing papers in PubMed.
- Article
- Distinct ppk-expressing Neurons Oppositely Regulate Sexual Receptivity in Drosophila Females.Neuroscience bulletin · 2026Article
- A positive feedback loop between sensory and octopaminergic neurons underlies nociceptive plasticity in Drosophila larvae.PLoS genetics · 2026Article
- Sequential formation ofScience advances · 2026Article
- Daily ultrastructural remodeling of clock neurons.Current biology : CB · 2025Article
- Neuropeptide-mediated synaptic plasticity regulates context-dependent mating behaviors in Drosophila.PLoS biology · 2025Article
- A pair of dopaminergic neurons DAN-c1 mediateeLife · 2025Article
- Article
- Article
- Generation of Odorant Receptor-QF2 Knock-In Drivers for Improved Analysis of Olfactory Circuits in Drosophila.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Neural substrates of cold nociception ineLife · 2025Article
- The lives of cells, recorded.Nature reviews. Genetics · 2025Review
- A hormone-to-neuropeptide pathway inhibits sexual receptivity in immatureProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Monitoring Synapses via Trans-synaptic GFP Complementation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Four SpsP neurons are an integrating sleep regulation hub inScience advances · 2024Article
- Advances in the labelling and selective manipulation of synapses.Nature reviews. Neuroscience · 2024Review
- Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Cell-cell communication: new insights and clinical implications.Signal transduction and targeted therapy · 2024Review
- Transsynaptic BMP Signaling Regulates Fine-Scale Topography between Adjacent Sensory Neurons.eNeuro · 2024Article
- A brain-derived insulin signal encodes protein satiety for nutrient-specific feeding inhibition.Cell reports · 2024Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Determining the pattern of activity of individual connections within a neural circuit could provide insights into the computational processes that underlie brain function. Here, we develop new strategies to label active synapses by trans-synaptic fluorescence complementation in Drosophila. First, we demonstrate that a synaptobrevin-GRASP chimera functions as a powerful activity-dependent marker for synapses in vivo. Next, we create cyan and yellow variants, achieving activity-dependent, multi-colour fluorescence reconstitution across synapses (X-RASP). Our system allows for the first time retrospective labelling of synapses (rather than whole neurons) based on their activity, in multiple colours, in the same animal. As individual synapses often act as computational units in the brain, our method will promote the design of experiments that are not possible using existing techniques. Moreover, our strategies are easily adaptable to circuit mapping in any genetic system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.