ArticleCell stress & chaperones2016
Cold stress increases reactive oxygen species formation via TRPA1 activation in A549 cells.
Article in Cell stress & chaperones, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- The meiotic achilles' heel: vulnerability and resilience under environmental stress.Protoplasma · 2026Review
- Reactive Oxygen Species: Molecular Mechanisms, Cellular Targets, and Implications for Genomic Stability.BioMed research international · 2026Review
- Developing an IPF Prognostic Model and Screening for Key Genes Based on Cold Exposure-Related Genes Using Bioinformatics Approaches.Biomedicines · 2025Article
- Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress.Scientific reports · 2025Article
- Combined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model.Clinical oral investigations · 2024Article
- Biological action of bleaching agents on tooth structure: A review.Histology and histopathology · 2024Review
- Associations of short-term ambient temperature exposure with lung function in middle-aged and elderly people: A longitudinal study in China.Eco-Environment & Health · 2024Article
- Controlled Hypothermic Storage for Lung Preservation: Leaving the Ice Age Behind.Transplant international : official journal of the European Society for Organ Transplantation · 2024Review
- Effects of Allyl Isothiocyanate on Oxidative and Inflammatory Stress in Type 2 Diabetic Rats.Molecules (Basel, Switzerland) · 2022Article
- Article
- Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells.International journal of molecular sciences · 2021Article
- Altered Induction of Reactive Oxygen Species by X-rays in Hematopoietic Cells of C57BL/6-Tg (CAG-EGFP) Mice.International journal of molecular sciences · 2021Article
- Effects of norepinephrine on colonic tight junction protein expression during heat stress.Experimental and therapeutic medicine · 2021Article
- Roles of Phase Separation for Cellular Redox Maintenance.Frontiers in genetics · 2021Review
- Article
- A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance inJournal of immunology research · 2020Article
- Genome-wide analysis of the cotton G-coupled receptor proteins (GPCR) and functional analysis of GTOM1, a novel cotton GPCR gene under drought and cold stress.BMC genomics · 2019Article
- Melatonin and Its Effects on Plant Systems.Molecules (Basel, Switzerland) · 2018Review
- Hydrogen Peroxide Induces Muscle Nociception via Transient Receptor Potential Ankyrin 1 Receptors.Anesthesiology · 2017Article
- Effects of Topical Icing on Inflammation, Angiogenesis, Revascularization, and Myofiber Regeneration in Skeletal Muscle Following Contusion Injury.Frontiers in physiology · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) are responsible for lung damage during inhalation of cold air. However, the mechanism of the ROS production induced by cold stress in the lung is still unclear. In this work, we measured the changes of ROS and the cytosolic Ca(2+) concentration ([Ca(2+)]c) in A549 cell. We observed that cold stress (from 20 to 5 °C) exposure of A549 cell resulted in an increase of ROS and [Ca(2+)]c, which was completely attenuated by removing Ca(2+) from medium. Further experiments showed that cold-sensing transient receptor potential subfamily member 1 (TRPA1) agonist (allyl isothiocyanate, AITC) increased the production of ROS and the level of [Ca(2+)]c in A549 cell. Moreover, HC-030031, a TRPA1 selective antagonist, significantly inhibited the enhanced ROS and [Ca(2+)]c induced by AITC or cold stimulation, respectively. Taken together, these data demonstrated that TRPA1 activation played an important role in the enhanced production of ROS induced by cold stress in A549 cell.
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