ArticlePlant & cell physiology2016
A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha.
Article in Plant & cell physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- The GENOMES UNCOUPLED1 protein has an ancient, highly conserved role but not in retrograde signalling.The New phytologist · 2022Article
- The renaissance and enlightenment of Marchantia as a model system.The Plant cell · 2022Article
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Construction of DNA Tools for Hyperexpression inACS synthetic biology · 2021Article
- I see the light! Fluorescent proteins suitable for cell wall/apoplast targeting inPlant direct · 2019Article
- Review
- Synthetic Botany.Cold Spring Harbor perspectives in biology · 2017Review
- Codon Optimization to Enhance Expression Yields Insights into Chloroplast Translation.Plant physiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Recently, the liverwort Marchantia polymorpha has received increasing attention as a basal plant model for multicellular studies. Its ease of handling, well-characterized plastome and proven protocols for biolistic plastid transformation qualify M. polymorpha as an attractive platform to study the evolution of chloroplasts during the transition from water to land. In addition, chloroplasts of M. polymorpha provide a convenient test-bed for the characterization of genetic elements involved in plastid gene expression due to the absence of mechanisms for RNA editing. While reporter genes have proven valuable to the qualitative and quantitative study of gene expression in chloroplasts, expression of green fluorescent protein (GFP) in chloroplasts of M. polymorpha has proven problematic. We report the design of a codon-optimized gfp varian, mturq2cp, which allowed successful expression of a cyan fluorescent protein under control of the tobacco psbA promoter from the chloroplast genome of M. polymorpha. We demonstrate the utility of mturq2cp in (i) early screening for transplastomic events following biolistic transformation of M. polymorpha spores; (ii) visualization of stromules as elements of plastid structure in Marchantia; and (iii) quantitative microscopy for the analysis of promoter activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.