ArticleBiomicrofluidics2015
On-chip recalcification of citrated whole blood using a microfluidic herringbone mixer.
Article in Biomicrofluidics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- The European guideline on management of major bleeding and coagulopathy following trauma: sixth edition.Critical care (London, England) · 2023Guideline
- Injury-on-a-chip for modelling microvascular trauma-induced coagulation.Lab on a chip · 2025Article
- [Update on point-of-care-based coagulation treatment : Systems, reagents, device-specific treatment algorithms].Die Anaesthesiologie · 2024Review
- Universal pre-mixing dry-film stickers capable of retrofitting existing microfluidics.Biomicrofluidics · 2023Article
- Coagulopathy management of multiple injured patients - a comprehensive literature review of the European guideline 2019.EFORT open reviews · 2022Review
- An "occlusive thrombosis-on-a-chip" microfluidic device for investigating the effect of anti-thrombotic drugs.Lab on a chip · 2021Article
- Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature.Annual review of biomedical engineering · 2021Review
- Thromboinflammation Model-on-A-Chip by Whole Blood Microfluidics on Fixed Human Endothelium.Diagnostics (Basel, Switzerland) · 2021Article
- Getting a good view:Platelets · 2020Review
- Technical Model of Micro Electrical Discharge Machining (EDM) Milling Suitable for Bottom Grooved Micromixer Design Optimization.Micromachines · 2020Article
- Shear-induced platelet aggregation: 3D-grayscale microfluidics for repeatable and localized occlusive thrombosis.Biomicrofluidics · 2019Article
- The European guideline on management of major bleeding and coagulopathy following trauma: fifth edition.Critical care (London, England) · 2019Article
- Evaluation of a microfluidic flow assay to screen for von Willebrand disease and low von Willebrand factor levels.Journal of thrombosis and haemostasis : JTH · 2018Article
- A Numerical Research of Herringbone Passive Mixer at Low Reynold Number Regime.Micromachines · 2017Article
- Enhanced Fibrinolysis with Magnetically Powered Colloidal Microwheels.Small (Weinheim an der Bergstrasse, Germany) · 2017Article
- Temperature effects on the activity, shape, and storage of platelets from 13-lined ground squirrels.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Article
- Flow chamber and microfluidic approaches for measuring thrombus formation in genetic bleeding disorders.Platelets · 2017Review
- Rapid, Self-driven Liquid Mixing on Open-Surface Microfluidic Platforms.Scientific reports · 2017Article
- Article
- A microfluidic model of hemostasis sensitive to platelet function and coagulation.Cellular and molecular bioengineering · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In vitro assays of platelet function and coagulation are typically performed in the presence of an anticoagulant. The divalent cation chelator sodium citrate is among the most common because its effect on coagulation is reversible upon reintroduction of divalent cations. Adding divalent cations into citrated blood by batch mixing leads to platelet activation and initiation of coagulation after several minutes, thus limiting the time blood can be used before spontaneously clotting. In this work, we describe a herringbone microfluidic mixer to continuously introduce divalent cations into citrated blood. The mixing ratio, defined as the ratio of the volumetric flow rates of citrated blood and recalcification buffer, can be adjusted by changing the relative inlet pressures of these two solutions. This feature is useful in whole blood assays in order to account for differences in hematocrit, and thus viscosity. The recalcification process in the herringbone mixer does not activate platelets. The advantage of this continuous mixing approach is demonstrated in microfluidic vascular injury model in which platelets and fibrin accumulate on a collagen-tissue factor surface under flow. Continuous recalcification with the herringbone mixer allowed for flow assay times of up to 30 min, more than three times longer than the time achieved by batch recalcification. This continuous mixer allows for measurements of thrombus formation, remodeling, and fibrinolysis in vitro over time scales that are relevant to these physiological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.