ArticleeLife2015
Untwisting the Caenorhabditis elegans embryo.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- Review
- Deep neural networks to register and annotate cells in moving and deforming nervous systems.eLife · 2026Article
- Neuroprotective properties of transition metal dichalcogenide nanoflowers alleviate acute and chronic neurological conditions linked to mitochondrial dysfunction.The Journal of biological chemistry · 2025Article
- Multiscale fluorescence imaging of living samples.Histochemistry and cell biology · 2022Review
- Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Toward a more accurate 3D atlas of C. elegans neurons.BMC bioinformatics · 2022Article
- Delineating the mechanisms and design principles ofComputational and structural biotechnology journal · 2022Review
- An Exact Hypergraph Matching algorithm for posture identification in embryonic C. elegans.PloS one · 2022Article
- From whole organism to ultrastructure: progress in axonal imaging for decoding circuit development.Development (Cambridge, England) · 2021Review
- Raising the Connectome: The Emergence of Neuronal Activity and Behavior inFrontiers in cellular neuroscience · 2020Article
- Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction.Nature methods · 2019Review
- Transforming the development and dissemination of cutting-edge microscopy and computation.Nature methods · 2019Article
- Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in AgingACS chemical biology · 2019Article
- Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution.Journal of visualized experiments : JoVE · 2019Article
- A high-content imaging approach to profileDevelopment (Cambridge, England) · 2019Article
- AceTree: a major update and case study in the long term maintenance of open-source scientific software.BMC bioinformatics · 2018Article
- Automated C. elegans embryo alignments reveal brain neuropil position invariance despite lax cell body placement.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
Abstract
The nematode Caenorhabditis elegans possesses a simple embryonic nervous system with few enough neurons that the growth of each cell could be followed to provide a systems-level view of development. However, studies of single cell development have largely been conducted in fixed or pre-twitching live embryos, because of technical difficulties associated with embryo movement in late embryogenesis. We present open-source untwisting and annotation software (http://mipav.cit.nih.gov/plugin_jws/mipav_worm_plugin.php) that allows the investigation of neurodevelopmental events in late embryogenesis and apply it to track the 3D positions of seam cell nuclei, neurons, and neurites in multiple elongating embryos. We also provide a tutorial describing how to use the software (Supplementary file 1) and a detailed description of the untwisting algorithm (Appendix). The detailed positional information we obtained enabled us to develop a composite model showing movement of these cells and neurites in an 'average' worm embryo. The untwisting and cell tracking capabilities of our method provide a foundation on which to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.