ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2015
Evaluation of Presurgical Skin Preparation Agents in African Clawed Frogs (Xenopus laevis).
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Analysis of Gross and Histopathologic Changes from Repeated Celiotomies in African Clawed Frogs (Xenopus laevis).Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Topical delivery of meropenem via spanlastic carbopol gel:Frontiers in pharmacology · 2025Article
- Health Monitoring for Laboratory Salamanders.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Molecular and culture-based assessment of the microbiome in a zebrafish (Danio rerio) housing system during set-up and equilibration.Animal microbiome · 2021Article
- Article
- Antimicrobial Susceptibility ofComparative medicine · 2021Article
- Using Waterless Alcohol-based Antiseptic for Skin Preparation and Active Thermal Support in Laboratory Rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Comparison of Two Hair Removal Methods in Sprague-Dawley Rats (Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Diagnosis, Surveillance and Management ofComparative medicine · 2020Article
- Comparison of Aqueous and Alcohol-based Agents for Presurgical Skin Preparation Methods in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2018Article
- Iron oxide nanoparticles can cross plasma membranes.Scientific reports · 2017Article
- Quantification of Induced Hypothermia from Aseptic Scrub Applications during Rodent Surgery Preparation.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Recommendations for Health Monitoring and Reporting for Zebrafish Research Facilities.Zebrafish · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Despite the routine collection of oocytes from African clawed frogs (Xenopus laevis) for use in research, few studies have evaluated methods for preparing their skin for surgery. We evaluated 3 skin preparatory agents by examining their antibacterial efficacy and the gross and microscopic appearance of Xenopus skin after exposure. Frogs (n = 14) were sedated and treated (contact time, 10 min) with 0.9% sterile NaCl on one-half of the ventrum and with 0.5% povidone-iodine or 0.75% chlorhexidine on the other half. Bacterial cultures were obtained before and after skin treatment; bacteria were identified by mass spectrometry. To assess inflammation and degenerative changes, the incision sites were photographed and biopsied at 0, 1, and 7 d after surgery. We isolated at least 22 genera of bacteria from the skin of our frog population (mean ± SE, 5.21 ± 0.82 genera per frog). Iodine (2.00 ± 0.44 genera) and chlorhexidine (0.29 ± 0.76 genera) both had greater antimicrobial activity than did saline. Skin erythema did not correlate with treatment group. Histologic evidence of epidermal degeneration and necrosis was greater on days 1 and 7 after chlorhexidine treatment than after iodine or saline. In addition, frogs treated with chlorhexidine had a higher incidence of clinical illness associated with the exposure site. In summary, although chlorhexidine has adequate antimicrobial activity against organisms on X. laevis skin, it leads to skin damage and subsequent clinical complications. We therefore do not recommend chlorhexidine as a preoperative preparation agent in Xenopus.
Indexed as
Identifiers
26632790PMC4671796W2443440602What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.