ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2015
Intraperitoneal Injection of Ethanol for the Euthanasia of Laboratory Mice (Mus musculus) and Rats (Rattus norvegicus).
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation.Redox report : communications in free radical research · 2025Article
- Dysregulation of Central-Medial Amygdala Histone Modifiers in Preclinical Models of Ethanol Exposure.Addiction biology · 2025Review
- Role of Allium cepa and Allium sativum extracts in oxidative stress, sperm parameters and histological abnormalities induced by deep-frying oil in testis.Scientific reports · 2025Article
- Genetic tools that target mechanoreceptors produce reliable labeling of bladder afferents and altered mechanosensation.American journal of physiology. Renal physiology · 2025Article
- Therapeutic role of mesenchymal stem cell-derived extracellular vesicles in neuroinflammation and cognitive dysfunctions induced by binge-like ethanol treatment in adolescent mice.CNS neuroscience & therapeutics · 2023Article
- Deep-frying palm olein oil-fried street falafel induces testicular toxicity in rats.Toxicology reports · 2023Article
- Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma.Bulletin of experimental biology and medicine · 2023Article
- The prospective effect of fucoidan on splenic dysfunction caused by oxaliplatin in male rats through endoplasmic stress dynamics.Scientific reports · 2022Article
- Linking mitochondrial dynamics and fertility: promoting fertility by phoenixin through modulation of ovarian expression of GnRH receptor and mitochondrial dynamics proteins DRP-1 and Mfn-2.Pflugers Archiv : European journal of physiology · 2022Article
- Intrahepatic Injection of Sodium Pentobarbital as an Alternative to Intraperitoneal Injection for the Euthanasia of Rats (Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Rapid SABRE Catalyst Scavenging Using Functionalized Silicas.Molecules (Basel, Switzerland) · 2022Article
- Article
- Role of mTOR-regulated autophagy in spine pruning defects and memory impairments induced by binge-like ethanol treatment in adolescent mice.Brain pathology (Zurich, Switzerland) · 2021Article
- Review of Rodent Euthanasia Methods.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Review
- Evaluating Intrahepatic and Intraperitoneal Sodium Pentobarbital or Ethanol for Mouse Euthanasia.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Review of Intraperitoneal Injection of Sodium Pentobarbital as a Method of Euthanasia in Laboratory Rodents.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Review
- Intraperitoneal injection of sodium pentobarbital has the potential to elicit pain in adult rats (Rattus norvegicus).PloS one · 2020Article
- Feifukang ameliorates pulmonary fibrosis by inhibiting JAK-STAT signaling pathway.BMC complementary and alternative medicine · 2018Article
- In vitro antioxidant and cholinesterase inhibitory activities of Elatostema papillosum leaves and correlation with their phytochemical profiles: a study relevant to the treatment of Alzheimer's disease.BMC complementary and alternative medicine · 2018Article
- Intraperitoneal Administration of Ethanol as a Means of Euthanasia for Neonatal Mice (Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compassion, professional ethics, and public sensitivity require that animals are euthanized humanely and appropriately under both planned and emergent situations. According to the 2013 AVMA Guidelines for the Euthanasia of Animals, intraperitoneal injection of ethanol is "acceptable with conditions" for use in mice. Because only limited information regarding this technique is available, we sought to evaluate ethanol by using ECG and high-definition video recording. Mice (n = 85) and rats (n = 16) were treated with intraperitoneal ethanol (70% or 100%), a positive-control agent (pentobarbital-phenytoin combination [Pe/Ph]), or a negative-control agent (saline solution). After injection, animals were assessed for behavioral and physiologic responses. Pain-assessment techniques in mice demonstrated that intraperitoneal injection of ethanol was not more painful than was intraperitoneal Pe/Ph. Median time to loss of consciousness for all mice that received ethanol or Pe/Ph was 45 s. Median time to respiratory arrest was 2.75, 2.25, and 2.63 min, and time (mean ± SE) to cardiac arrest was 6.04 ± 1.3, 2.96 ± 0.6, and 4.03 ± 0.5 min for 70% ethanol, 100% ethanol, and Pe/Ph, respectively. No mouse that received ethanol or Pe/Ph regained consciousness. Although successful in mice, intraperitoneal ethanol at the doses tested (9.2 to 20.1 g/kg) was unsuitable for euthanasia of rats (age, 7 to 8 wk) because of the volume needed and prolonged time to respiratory effects. For mice, intraperitoneal injection of 70% or 100% ethanol induced rapid and irreversible loss of consciousness, followed by death, and should be considered as "acceptable with conditions."
Indexed as
Identifiers
26632787PMC4671793What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.