ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2015
Interactions Between Housing Density and Ambient Temperature in the Cage Environment: Effects on Mouse Physiology and Behavior.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Port of Choice: Induction Chamber Inlet Position Affects Speed of Isoflurane Induction in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Guidelines for diet-induced models of cardiometabolic syndrome.American journal of physiology. Heart and circulatory physiology · 2025Review
- Longitudinal Study of Changes in Ammonia, Carbon Dioxide, Humidity and Temperature in Individually Ventilated Cages Housing Female and Male C57BL/6N Mice during Consecutive Cycles of Weekly and Bi-Weekly Cage Changes.Animals : an open access journal from MDPI · 2024Article
- Quantifying the Impact of Co-Housing on Murine Aging Studies.bioRxiv : the preprint server for biology · 2024Article
- Impact of housing temperature on adipose tissue HDAC9 expression and adipogenic differentiation in high fat-fed mice.Obesity (Silver Spring, Md.) · 2024Article
- Rodent models for oral microbiome research: considerations and challenges- a mini review.Frontiers in oral health · 2024Review
- Novel Husbandry Practices Result in Rapid Rates of Growth and Sexual Maturation Without Impacting Adult Behavior in the Blind Mexican Cavefish.Zebrafish · 2023Article
- How murine models of human disease and immunity are influenced by housing temperature and mild thermal stress.Temperature (Austin, Tex.) · 2023Review
- Effect of Different Ambient Temperatures on Reproductive Outcome and Stress Level of Lactating Females in Two Mouse Strains.Animals : an open access journal from MDPI · 2022Article
- Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The "Goldilocks Solution".Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Effects of Laboratory Housing Conditions on Core Temperature and Locomotor Activity in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Dietary Options for Rodents in the Study of Obesity.Nutrients · 2020Review
- Review
- Individual housing of male C57BL/6J mice after weaning impairs growth and predisposes for obesity.PloS one · 2020Article
- Recommended housing densities for research mice: filling the gap in data-driven alternatives.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Review
- Manipulation of Ambient Housing Temperature To Study the Impact of Chronic Stress on Immunity and Cancer in Mice.Journal of immunology (Baltimore, Md. : 1950) · 2019Review
- Immune Relevant and Immune Deficient Mice: Options and Opportunities in Translational Research.ILAR journal · 2018Review
- Low temperature decreases bone mass in mice: Implications for humans.American journal of physical anthropology · 2018Article
- The mouse thermoregulatory system: Its impact on translating biomedical data to humans.Physiology & behavior · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine how housing density and ambient temperature interact to influence the physiology and behavior of mice, we systematically varied housing density (1 to 5 mice per cage) and ambient temperature (22, 26, or 30 °C) and measured effects on body weight, food intake, diurnal patterns of locomotor activity and core temperature, fecal corticosterone, and serum cytokine and adipokine panels. Temperatures inside cages housing 5 mice were 1 to 2 °C higher than the ambient temperature. As the housing density decreased, in-cage temperatures began to fall at a density of 2 or 3 mice per cage and did not differ from ambient temperature at 1 mouse per cage. Ambient temperature, but not housing density, significantly affected food intake. Although neither ambient temperature nor housing density affected core temperature or activity, hyperthermia and behavioral activation occurred during the 12-h period after cage change. Fecal concentrations of corticosterone metabolites and serum cytokines, chemokines, insulin, and leptin were not influenced by cage density and were only sporadically influenced by ambient temperature. Our data document that the number of mice housed per cage influences the intracage environmental conditions and that ambient temperature influences food intake even when temperatures are within or near recommended or thermoneutral ranges. We conclude that investigators should be cautious when changing the number of mice housed in a cage over the course of a study, because doing so significantly alters the cage environment to which remaining mice are exposed.
Indexed as
Identifiers
26632780PMC4671786W2412741006What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.