ArticleNature communications2015
Gln40 deamidation blocks structural reconfiguration and activation of SCF ubiquitin ligase complex by Nedd8.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Nanopore-based massively parallel sensing for peptide profiling and protein identification.Nature communications · 2026Article
- Kinetic principles of chemical cross-link formation for protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ubiquitin-targeted bacterial effectors: rule breakers of the ubiquitin system.The EMBO journal · 2023Review
- An expanded lexicon for the ubiquitin code.Nature reviews. Molecular cell biology · 2023Review
- E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity.Molecular cell · 2023Article
- Developing a Targeted Quantitative Strategy for Sulfoxide-Containing MS-Cleavable Cross-Linked Peptides to Probe Conformational Dynamics of Protein Complexes.Analytical chemistry · 2022Article
- Cullin-RING Ubiquitin Ligase Regulatory Circuits: A Quarter Century Beyond the F-Box Hypothesis.Annual review of biochemistry · 2021Review
- Expression and purification of functional recombinant CUL2•RBX1 from E. coli.Scientific reports · 2021Article
- NEDD8 and ubiquitin ligation by cullin-RING E3 ligases.Current opinion in structural biology · 2021Review
- NEDD8 Deamidation Inhibits Cullin RING Ligase Dynamics.Frontiers in immunology · 2021Article
- Bacterial virulence mediated by orthogonal post-translational modification.Nature chemical biology · 2020Review
- Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Post-translational modification and protein sorting to small extracellular vesicles including exosomes by ubiquitin and UBLs.Cellular and molecular life sciences : CMLS · 2019Review
- Surface Glycoproteomic Analysis Reveals That Both Unique and Differential Expression of Surface Glycoproteins Determine the Cell Type.Analytical chemistry · 2019Article
- Probing HMolecular & cellular proteomics : MCP · 2019Article
- Data-independent Acquisition Improves Quantitative Cross-linking Mass Spectrometry.Molecular & cellular proteomics : MCP · 2019Article
- Quantitative cross-linking/mass spectrometry to elucidate structural changes in proteins and their complexes.Nature protocols · 2019Article
- Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology.Nature structural & molecular biology · 2018Review
- Protein Dynamics in Solution by Quantitative Crosslinking/Mass Spectrometry.Trends in biochemical sciences · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby interfering with cellular functions. Despite extensive structural studies on CRLs, the molecular mechanism by which Nedd8 Gln40 deamidation affects CRL functions remains unclear. We apply a new quantitative cross-linking mass spectrometry approach to characterize three different types of full-length human Cul1-Rbx1 complexes and uncover major Nedd8-induced structural rearrangements of the CRL1 catalytic core. More importantly, we find that those changes are not induced by Nedd8(Q40E) conjugation, indicating that the subtle change of a single Nedd8 amino acid is sufficient to revert the structure of the CRL catalytic core back to its unmodified form. Our results provide new insights into how neddylation regulates the conformation and activity of CRLs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.