ArticleNature communications2015
Optogenetic mutagenesis in Caenorhabditis elegans.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Microbial Lipopolysaccharide Regulates Host Development Through Insulin/IGF-1 Signaling.International journal of molecular sciences · 2025Article
- Loss of neuropeptidergic regulation of cholinergic transmission induces homeostatic compensation in muscle cells to preserve synaptic strength.PLoS biology · 2025Article
- Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.Molecular genetics and genomics : MGG · 2021Review
- The mRNA Decay Factor CAR-1/LSM14 Regulates Axon Regeneration via Mitochondrial Calcium Dynamics.Current biology : CB · 2020Article
- Light-induced oxidant production by fluorescent proteins.Free radical biology & medicine · 2018Review
- Blue-Light Receptors for Optogenetics.Chemical reviews · 2018Review
- Feeding state regulates pheromone-mediated avoidance behavior via the insulin signaling pathway inThe EMBO journal · 2018Article
- Rapid Integration of Multi-copy Transgenes Using Optogenetic Mutagenesis inG3 (Bethesda, Md.) · 2018Article
- Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.Free radical biology & medicine · 2018Article
- Review
- Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.Frontiers in physiology · 2017Review
- Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.Nature reviews. Genetics · 2017Review
- Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans.Journal of visualized experiments : JoVE · 2016Article
- Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans.Scientific reports · 2016Article
- Targeted genome engineering inCell & bioscience · 2016Review
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Authors and funding
2 authors.
Funding
Abstract
Reactive oxygen species (ROS) can modify and damage DNA. Here we report an optogenetic mutagenesis approach that is free of toxic chemicals and easy to perform by taking advantage of a genetically encoded ROS generator. This method relies on the potency of ROS generation by His-mSOG, the mini singlet oxygen generator, miniSOG, fused to a histone. Caenorhabditis elegans expressing His-mSOG in the germline behave and reproduce normally, without photoinduction. Following exposure to blue light, the His-mSOG animals produce progeny with a wide range of heritable phenotypes. We show that optogenetic mutagenesis by His-mSOG induces a broad spectrum of mutations including single-nucleotide variants (SNVs), chromosomal deletions, as well as integration of extrachromosomal transgenes, which complements those derived from traditional chemical or radiation mutagenesis. The optogenetic mutagenesis expands the toolbox for forward genetic screening and also provides direct evidence that nuclear ROS can induce heritable and specific genetic mutations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.