Evidence map›Paper›PMID 26632206›Full record

ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2016

Comparison of natural crosslinking agents for the stabilization of xenogenic articular cartilage.

Amanda Pinheiro, Avery Cooley, Jun Liao, Rajkumar Prabhu, Steven Elder

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 79 citations in OpenAlex.

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  17. Evaluation of genipin for stabilization of decellularized porcine cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Amanda PinheiroDepartment of Biomedical Engineering, The University of Akron, Akron, Ohio, 44325.
Avery CooleyDepartment of Pathobiology and Population Medicine, Mississippi State University, Starkville, Mississippi, 39762.
Jun LiaoDepartment of Agricultural and Biological Engineering, Mississippi State University, Starkville, Mississippi, 39762.
Rajkumar PrabhuDepartment of Agricultural and Biological Engineering, Mississippi State University, Starkville, Mississippi, 39762.
Steven ElderDepartment of Agricultural and Biological Engineering, Mississippi State University, Starkville, Mississippi, 39762.
Mississippi State University · USUniversity of Akron · US

Funding

Development of an Ostoechondral Xenograft for Articular Cartilage RepairR15AR066926 · NIAMS · MISSISSIPPI STATE UNIVERSITY · PI ELDER, STEVEN HOWARD · 2015 to 2015
$367k
NIAMS NIH HHS R15 AR066926
6 · The paper itself

Abstract

Osteochondral xenografts are potentially inexpensive, widely available alternatives to fresh allografts. However, antigen removal from xenogenic cartilage may damage the extracellular matrix and reduce compressive stiffness. Non-crosslinked xenogenic cartilage may also undergo rapid enzymatic degradation in vivo. We hypothesized that natural crosslinking agents could be used in place of glutaraldehyde to improve the mechanical properties and enzymatic resistance of decellularized cartilage. This study compared the effects of genipin (GNP), proanthocyanidin (PA), and epigallocatechin gallate (EGCG), on the physical and mechanical properties of decellularized porcine cartilage. Glutaraldehyde (GA) served as a positive control. Porcine articular cartilage discs were decellularized in 2% sodium dodecyl sulfate and DNase I followed by fixation in 0.25% GNP, 0.25% PA, 0.25% EGCG, or 2.5% GA. Decellularization decreased DNA by 15% and GAG by 35%. For natural crosslinkers, the average degree of crosslinking ranged from approximately 50% (EGCG) to 78% (GNP), as compared to 83% for the GA control. Among the natural crosslinkers, only GNP significantly affected the disc diameter, and shrinkage was under 2%. GA fixation had no significant effect on disc diameter. Decellularization decreased aggregate modulus; GA and GNP, but not EGCG and PA, were able to restore it to its original level. GNP, PA, and GA conferred a similar, almost complete resistance to collagenase degradation. EGCG also conferred substantial resistance but to a lesser degree. Overall, the data support our hypothesis and suggest that natural crosslinkers may be suitable alternatives to glutaraldehyde for stabilization of decellularized cartilage. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1037-1046, 2016.

Indexed as

AnimalsCartilageCartilage, ArticularCatechinCollagenasesCross-Linking ReagentsDrug Evaluation, PreclinicalGrape Seed ExtractHeterograftsIridoidsProanthocyanidinsSwineCatechinCollagenasesCross-Linking Reagentsepigallocatechin gallategenipinGrape Seed ExtractGrape Seed ProanthocyanidinsIridoidsProanthocyanidinscartilagecrosslinkingepigallocatechin gallategenipinproanthocyanidinxenograft

Identifiers

PMID26632206
PMCPMC6791374
OpenAlexW2187784371

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.