ArticleScientific reports2015
Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- High-throughput strategies for monoclonal antibody screening: advances and challenges.Journal of biological engineering · 2025Review
- Protein sequence modelling with Bayesian flow networks.Nature communications · 2025Article
- Monoclonal antibodies: From magic bullet to precision weapon.Molecular biomedicine · 2024Review
- Simultaneous Maturation of Single Chain Antibody Stability and Affinity by CHO Cell Display.Bioengineering (Basel, Switzerland) · 2022Article
- Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.Nature biotechnology · 2022Article
- Disulfide-compatible phage-assisted continuous evolution in the periplasmic space.Nature communications · 2021Article
- Bivalent antibody pliers inhibit β-tryptase by an allosteric mechanism dependent on the IgG hinge.Nature communications · 2020Article
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- Understanding and overcoming trade-offs between antibody affinity, specificity, stability and solubility.Biochemical engineering journal · 2018Article
- Efficient affinity maturation of antibody variable domains requires co-selection of compensatory mutations to maintain thermodynamic stability.Scientific reports · 2017Article
- Facile Affinity Maturation of Antibody Variable Domains Using Natural Diversity Mutagenesis.Frontiers in immunology · 2017Article
- Gene-Specific Substitution Profiles Describe the Types and Frequencies of Amino Acid Changes during Antibody Somatic Hypermutation.Frontiers in immunology · 2017Article
- Molecular dynamics simulations and docking enable to explore the biophysical factors controlling the yields of engineered nanobodies.Scientific reports · 2016Article
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4 authors.
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Abstract
Rapid identification of residues that influence antibody expression and thermostability is often needed to move promising therapeutics into the clinic. To establish a method that can assess small expression differences, we developed a Bacterial Antibody Display (BAD) system that overcomes previous limitations, enabling the use of full-length formats for antibody and antigen in a live cell setting. We designed a unique library of individual framework variants using natural diversity introduced by somatic hypermutation, and screened half-antibodies for increased expression using BAD. We successfully identify variants that dramatically improve expression yields and in vitro thermostability of two therapeutically relevant antibodies in E. coli and mammalian cells. While we study antibody expression, bacterial display can now be expanded to examine the processes of protein folding and translocation. Additionally, our natural library design strategy could be applied during antibody humanization and library design for in vitro display methods to maintain expression and formulation stability.
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